Steroid receptor coactivator-1 coactivates activating protein-1- mediated transactivations through interaction with the c-Jun and c-Fos subunits

Soo-Kyung Lee, Han Jong Kim, Soon Young Na, Tae Sung Kim, Hueng Sik Choi, Suhn Young Im, Jae Lee

Research output: Contribution to journalArticle

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Abstract

Steroid receptor coactivator-1 (SRC-1) specifically bound to the transcription factor AP-1 subunits c-Jun and c-Fos, as demonstrated by the yeast two-hybrid tests and glutathione S-transferase pull down assays. The c- Jun and c-Fos binding sites were localized to the C-terminal subregion of SRC-1 (amino acids 1101-1441) that encompasses the previously described histone acetyltransferase and receptor-binding domains. In mammalian cells, SRC-1, similar to the previous results with CBP-p300 (Arias, J., Alberts, A. S., Brindle, P., Claret, F. X., Smeal, T., Karin, M., Feramisco, J., and Montminy, M. (1994) Nature 370, 226-229; Bannister, A. J., and Kouzarides, T. (1995) EMBO J. 14, 4758-4762), potentiated the AP-1-mediated transactivations in a dose-dependent manner and derepressed the mutual inhibitions between nuclear receptors and AP-1. Furthermore, coexpression of p300 further enhanced this SRC-1-potentiated level of transactivations. Thus, we concluded that at least two distinct coactivator molecules may cooperate to regulate AP-1-dependent transactivations and mediate transrepression between AP-1 and nuclear receptors in vivo.

Original languageEnglish (US)
Pages (from-to)16651-16654
Number of pages4
JournalJournal of Biological Chemistry
Volume273
Issue number27
DOIs
StatePublished - Jul 3 1998
Externally publishedYes

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Nuclear Receptor Coactivator 1
Transcription Factor AP-1
Transcriptional Activation
Cytoplasmic and Nuclear Receptors
Proteins
Histone Acetyltransferases
Glutathione Transferase
Yeast
Assays
Yeasts
Binding Sites
Cells
Amino Acids
Molecules

ASJC Scopus subject areas

  • Biochemistry

Cite this

Steroid receptor coactivator-1 coactivates activating protein-1- mediated transactivations through interaction with the c-Jun and c-Fos subunits. / Lee, Soo-Kyung; Kim, Han Jong; Na, Soon Young; Kim, Tae Sung; Choi, Hueng Sik; Im, Suhn Young; Lee, Jae.

In: Journal of Biological Chemistry, Vol. 273, No. 27, 03.07.1998, p. 16651-16654.

Research output: Contribution to journalArticle

Lee, Soo-Kyung ; Kim, Han Jong ; Na, Soon Young ; Kim, Tae Sung ; Choi, Hueng Sik ; Im, Suhn Young ; Lee, Jae. / Steroid receptor coactivator-1 coactivates activating protein-1- mediated transactivations through interaction with the c-Jun and c-Fos subunits. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 27. pp. 16651-16654.
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