Steroid receptor coactivator-1 and its family members differentially regulate transactivation by the tumor suppressor protein p53

Soo Kyung Lee, Han Jong Kim, Jung Woo Kim, Jae Woon Lee

Research output: Contribution to journalArticlepeer-review

66 Scopus citations

Abstract

The tumor suppressor protein p53 exerts its cell cycle-regulatory effects through its ability to function as a sequence-specific DNA-binding transcription factor. Herein, we show that p53 physically interacts with specific subregions of steroid receptor coactivator-1 (SRC-1) and its family members, p/ClP (p300/CBP interacting protein), xSRC-3, and AIB1 (amplified in breast cancer), originally isolated as transcription coactivators of nuclear receptors, as demonstrated by the yeast and mammalian two-hybrid tests as well as glutathione S-transferase pull-down assays. Interestingly, cotransfection of HeLa cells with SRC-1- or p/ClP expression vector potentiated the p53-mediated transactivation, whereas AIB1 and xSRC-3 were repressive. All of these SRC-1 members, however, similarly stimulated transactivation mediated by nuclear receptors and AP-1, as previously described. These results suggest that SRC-1 and its family members may differentially modulate the p53 transactivation in vivo.

Original languageEnglish (US)
Pages (from-to)1924-1933
Number of pages10
JournalMolecular Endocrinology
Volume13
Issue number11
DOIs
StatePublished - 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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