Steroid control of acute middle ear inflammation in a mouse model

Carol Macarthur, Jacqueline M. DeGagne, J. Beth Kempton, Dennis Trune

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Objective: To investigate steroids for their potential for therapeutic approaches to control otitis media. Glucocorticoids and mineralocorticoids have differential effects on inflammation and fluid absorption, but little is known of their control of middle and inner ear manifestations of acute otitis media. Design: Both glucocorticoid (prednisolone and dexamethasone) and mineralocorticoid (aldosterone and fludrocortisone) steroids were investigated for their ability to reduce inflammatory symptoms in a mouse otitis media model. Setting: Academic medical center. Subjects: Acute inflammation was induced by transtympanic injection of heat killed Streptococcus pneumoniae to 100 BALB/c mice. Interventions: Twenty mice in each experimental group (prednisolone, dexamethasone, aldosterone, and fludrocortisone) were given a steroid in their drinking water the day before inoculation, and these treatments were continued until the mice were killed for histologic examination. Twenty control mice were treated with water only. Main Outcome Measures: Histologic measure of inflammation: middle ear fluid, inflammatory cell number, and tympanic membrane thickness. Results: Histologic middle ear morphometrics showed significant steroid effects at both 3 and 5 days in reduction of fluid area, cell number, and tympanic membrane thickness. Conclusions: Glucocorticoids were most effective in controlling inflammation. Interestingly, the mineralocorticoids were also effective in reducing the inflammatory response at 5 days, suggesting that their fluid transport function helped clear disease. Thus, steroid control of middle ear disease may be useful in alleviating symptoms faster and reducing the risk to the inner ear.

Original languageEnglish (US)
Pages (from-to)453-457
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume135
Issue number5
DOIs
StatePublished - May 2009

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Otitis Media
Mineralocorticoids
Steroids
Middle Ear
Fludrocortisone
Glucocorticoids
Tympanic Membrane
Inner Ear
Prednisolone
Inflammation
Aldosterone
Dexamethasone
Cell Count
Cell Membrane
Ear Diseases
Streptococcus pneumoniae
Drinking Water
Hot Temperature
Outcome Assessment (Health Care)
Injections

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery
  • Medicine(all)

Cite this

Steroid control of acute middle ear inflammation in a mouse model. / Macarthur, Carol; DeGagne, Jacqueline M.; Kempton, J. Beth; Trune, Dennis.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 135, No. 5, 05.2009, p. 453-457.

Research output: Contribution to journalArticle

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