Stereotaxic surgical targeting of the nonhuman primate caudate and putamen: Gene therapy for huntington’s disease

Jodi McBride, Randall L. Clark

    Research output: Chapter in Book/Report/Conference proceedingChapter

    3 Citations (Scopus)

    Abstract

    Stereotaxic surgery is an invaluable tool to deliver a variety of gene therapy constructs to the nonhuman primate caudate and putamen in preclinical studies for the genetic, neurodegenerative disorder, Huntington’s disease (HD). Here we describe in detail how to perform this technique beginning with a pre-surgical magnetic resonance imaging scan to determine surgical coordinates followed by the stereotaxic surgical injection technique. In addition, we include methodology of a full necropsy including brain and peripheral tissue removal and a standard immunohistochemical technique to visualize the injected gene therapy agent.

    Original languageEnglish (US)
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Pages409-428
    Number of pages20
    Volume1382
    DOIs
    StatePublished - 2016

    Publication series

    NameMethods in Molecular Biology
    Volume1382
    ISSN (Print)10643745

    Fingerprint

    Putamen
    Huntington Disease
    Genetic Therapy
    Primates
    Inborn Genetic Diseases
    Neurodegenerative Diseases
    Magnetic Resonance Imaging
    Injections
    Brain

    Keywords

    • Huntington’s disease
    • Immunohistochemistry
    • Magnetic resonance imaging
    • Necropsy
    • Neurodegeneration
    • Nonhuman primate
    • Stereotaxic surgery

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

    Cite this

    McBride, J., & Clark, R. L. (2016). Stereotaxic surgical targeting of the nonhuman primate caudate and putamen: Gene therapy for huntington’s disease. In Methods in Molecular Biology (Vol. 1382, pp. 409-428). (Methods in Molecular Biology; Vol. 1382). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3271-9_29

    Stereotaxic surgical targeting of the nonhuman primate caudate and putamen : Gene therapy for huntington’s disease. / McBride, Jodi; Clark, Randall L.

    Methods in Molecular Biology. Vol. 1382 Humana Press Inc., 2016. p. 409-428 (Methods in Molecular Biology; Vol. 1382).

    Research output: Chapter in Book/Report/Conference proceedingChapter

    McBride, J & Clark, RL 2016, Stereotaxic surgical targeting of the nonhuman primate caudate and putamen: Gene therapy for huntington’s disease. in Methods in Molecular Biology. vol. 1382, Methods in Molecular Biology, vol. 1382, Humana Press Inc., pp. 409-428. https://doi.org/10.1007/978-1-4939-3271-9_29
    McBride J, Clark RL. Stereotaxic surgical targeting of the nonhuman primate caudate and putamen: Gene therapy for huntington’s disease. In Methods in Molecular Biology. Vol. 1382. Humana Press Inc. 2016. p. 409-428. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3271-9_29
    McBride, Jodi ; Clark, Randall L. / Stereotaxic surgical targeting of the nonhuman primate caudate and putamen : Gene therapy for huntington’s disease. Methods in Molecular Biology. Vol. 1382 Humana Press Inc., 2016. pp. 409-428 (Methods in Molecular Biology).
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