Stereoisomeric configuration of arabinitol in serum, urine, and tissues in invasive candidiasis

E. M. Bernard, B. Wong, D. Armstrong

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P < .001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis.

Original languageEnglish (US)
Pages (from-to)711-715
Number of pages5
JournalJournal of Infectious Diseases
Volume151
Issue number4
DOIs
StatePublished - 1985

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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