Abstract
Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P <.001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 711-715 |
| Number of pages | 5 |
| Journal | Journal of Infectious Diseases |
| Volume | 151 |
| Issue number | 4 |
| State | Published - 1985 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
- Public Health, Environmental and Occupational Health
Cite this
Stereoisomeric configuration of arabinitol in serum, urine, and tissues in invasive candidiasis. / Bernard, E. M.; Wong, Brian; Armstrong, D.
In: Journal of Infectious Diseases, Vol. 151, No. 4, 1985, p. 711-715.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Stereoisomeric configuration of arabinitol in serum, urine, and tissues in invasive candidiasis
AU - Bernard, E. M.
AU - Wong, Brian
AU - Armstrong, D.
PY - 1985
Y1 - 1985
N2 - Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P <.001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis.
AB - Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P <.001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis.
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M3 - Article
C2 - 3973418
AN - SCOPUS:0021923152
VL - 151
SP - 711
EP - 715
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -