TY - JOUR
T1 - Stereoisomeric configuration of arabinitol in serum, urine, and tissues in invasive candidiasis
AU - Bernard, E. M.
AU - Wong, B.
AU - Armstrong, D.
N1 - Funding Information:
Receivedfor publication July 2,1984, and in revised form October 17, 1984. This work was presented in part at the 23rd Interscience Conference on Antimicrobial Agents and Chemotherapy, held in Las Vegas, Nevada, in October 1983. Dr. Wong was supported in part by Junior Faculty Clinical Fellowship 695B from the American Cancer Society. Please address requests for reprints to Edward M. Bernard, Diagnostic Microbiology Laboratory, Department of Medicine, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, New York 10021. * Present address: Division of Infectious Diseases, Department of Internal Medicine, University of Cincinnati College of Medicine, Cincinnati, Ohio.
PY - 1985
Y1 - 1985
N2 - Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P < .001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis.
AB - Because routine analytical methods cannot differentiate D- from L-arabinitol, a combined microbiological and gas chromatographic method was developed to study the stereoisomeric configuration of the arabinitol in humans and rats with invasive candidiasis. D-Arabinitol was defined as the difference between arabinitol concentrations measured with and without incubation with 5.0 x 105 blastospores of Candida tropicalis strain CT 12 at 37 C for 24 hr. The yeast consumed at least 95% of the D-arabinitol and none of the L-arabinitol added to normal serum and urine. D-Arabinitol as a fraction of D,L-arabinitol was 0.43 ± 0.15 (mean ± SD) in the urine of 10 normal humans, 0.82 ± 0.12 in the serum or urine of five patients with cancer and invasive candidiasis (P < .001), and 1.0 in the kidneys of rats with candidiasis. Because most or all of the excess arabinitol in body fluids or tissues in candidiasis was the D isomer, which is produced by fungal metabolism, stereospecific quantitation of arabinitol should improve the sensitivity of this approach to diagnosis of candidiasis.
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U2 - 10.1093/infdis/151.4.711
DO - 10.1093/infdis/151.4.711
M3 - Article
C2 - 3973418
AN - SCOPUS:0021923152
VL - 151
SP - 711
EP - 715
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 4
ER -