Stereocilia membrane deformation: Implications for the gating spring and mechanotransduction channel

Richard J. Powers, Sitikantha Roy, Erdinc Atilgan, William E. Brownell, Sean X. Sun, Peter Barr-Gillespie, Alexander A. Spector

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In hair cells, although mechanotransduction channels have been localized to tips of shorter stereocilia of the mechanically sensitive hair bundle, little is known about how force is transmitted to the channel. Here, we use a biophysical model of the membrane-channel complex to analyze the nature of the gating spring compliance and channel arrangement. We use a triangulated surface model and Monte Carlo simulation to compute the deformation of the membrane under the action of tip link force. We show that depending on the gating spring stiffness, the compliant component of the gating spring arises from either the membrane alone or a combination of the membrane and a tether that connects the channel to the actin cytoskeleton. If a bundle is characterized by relatively soft gating springs, such as those of the bullfrog sacculus, the need for membrane reinforcement by channel tethering then depends on membrane parameters. With stiffer gating springs, such as those from rat outer hair cells, the channel must be tethered for all biophysically realistic parameters of the membrane. We compute the membrane forces (resultants), which depend on membrane tension, bending modulus, and curvature, and show that they can determine the fate of the channel.

Original languageEnglish (US)
Pages (from-to)201-210
Number of pages10
JournalBiophysical Journal
Volume102
Issue number2
DOIs
StatePublished - Jan 18 2012

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Stereocilia
Membranes
Ion Channels
Outer Auditory Hair Cells
Rana catesbeiana
Actin Cytoskeleton
Compliance

ASJC Scopus subject areas

  • Biophysics

Cite this

Stereocilia membrane deformation : Implications for the gating spring and mechanotransduction channel. / Powers, Richard J.; Roy, Sitikantha; Atilgan, Erdinc; Brownell, William E.; Sun, Sean X.; Barr-Gillespie, Peter; Spector, Alexander A.

In: Biophysical Journal, Vol. 102, No. 2, 18.01.2012, p. 201-210.

Research output: Contribution to journalArticle

Powers, Richard J. ; Roy, Sitikantha ; Atilgan, Erdinc ; Brownell, William E. ; Sun, Sean X. ; Barr-Gillespie, Peter ; Spector, Alexander A. / Stereocilia membrane deformation : Implications for the gating spring and mechanotransduction channel. In: Biophysical Journal. 2012 ; Vol. 102, No. 2. pp. 201-210.
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