Stepwise insertion and inversion of a type II signal anchor sequence in the ribosome-Sec61 translocon complex

Prasanna K. Devaraneni, Brian Conti, Yoshihiro Matsumura, Zhongying Yang, Arthur E. Johnson, William Skach

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In eukaryotic cells, the ribosome-Sec61 translocon complex (RTC) establishes membrane protein topology by cotranslationally partitioning nascent polypeptides into the cytosol, ER lumen, and lipid bilayer. Using photocrosslinking, collisional quenching, cysteine accessibility, and protease protection, we show that a canonical type II signal anchor (SA) acquires its topology through four tightly coupled and mechanistically distinct steps: (1) head-first insertion into Sec61α, (2) nascent chain accumulation within the RTC, (3) inversion from type I to type II topology, and (4) stable translocation of C-terminal flanking residues. Progression through each stage is induced by incremental increases in chain length and involves abrupt changes in the molecular environment of the SA. Importantly, type II SA inversion deviates from a type I SA at an unstable intermediate whose topology is controlled by dynamic interactions between the ribosome and translocon. Thus, the RTC coordinates SA topogenesis within a protected environment via sequential energetic transitions of the TM segment.

Original languageEnglish (US)
Pages (from-to)134-147
Number of pages14
JournalCell
Volume146
Issue number1
DOIs
StatePublished - Jul 8 2011

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Protein Sorting Signals
Anchors
Ribosomes
Topology
Cysteine Proteases
Lipid Bilayers
Eukaryotic Cells
Lipid bilayers
Cytosol
Membrane Proteins
Chain length
Head
Cysteine
Quenching
Peptide Hydrolases
Peptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Devaraneni, P. K., Conti, B., Matsumura, Y., Yang, Z., Johnson, A. E., & Skach, W. (2011). Stepwise insertion and inversion of a type II signal anchor sequence in the ribosome-Sec61 translocon complex. Cell, 146(1), 134-147. https://doi.org/10.1016/j.cell.2011.06.004

Stepwise insertion and inversion of a type II signal anchor sequence in the ribosome-Sec61 translocon complex. / Devaraneni, Prasanna K.; Conti, Brian; Matsumura, Yoshihiro; Yang, Zhongying; Johnson, Arthur E.; Skach, William.

In: Cell, Vol. 146, No. 1, 08.07.2011, p. 134-147.

Research output: Contribution to journalArticle

Devaraneni, PK, Conti, B, Matsumura, Y, Yang, Z, Johnson, AE & Skach, W 2011, 'Stepwise insertion and inversion of a type II signal anchor sequence in the ribosome-Sec61 translocon complex', Cell, vol. 146, no. 1, pp. 134-147. https://doi.org/10.1016/j.cell.2011.06.004
Devaraneni, Prasanna K. ; Conti, Brian ; Matsumura, Yoshihiro ; Yang, Zhongying ; Johnson, Arthur E. ; Skach, William. / Stepwise insertion and inversion of a type II signal anchor sequence in the ribosome-Sec61 translocon complex. In: Cell. 2011 ; Vol. 146, No. 1. pp. 134-147.
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