Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and independent mechanisms

Makoto Miyazaki, Agnieszka Dobrzyn, Weng Chi Man, Kiki Chu, Harini Sampath, Hyoun Ju Kim, James M. Ntambi

Research output: Contribution to journalArticle

198 Citations (Scopus)

Abstract

Stearoyl-CoA desaturase (SCD) synthesizes oleate necessary for the biosynthesis of triglycerides and other lipids. Mice with a targeted disruption of the SCD1 gene are deficient in tissue oleate and have reduced expression of the sterol regulatory element-binding protein (SREBP) and its target genes. The SREBP-1c isoform is a known mediator of insulin action on hepatic gene expression, but its transcriptional effects due to glucose or fructose are still unclear. We found that fructose compared with glucose is a stronger inducer of SREBP-1c and lipogenic gene expression, causing a dramatic increase in hepatic triglyceride levels. However, when fed to the SCD1-/- mice, fructose failed to induce SREBP-1 or lipogenic genes and the triglyceride levels were not increased. Instead fructose feeding caused a decrease in hepatic glycogen and plasma glucose levels. The induction of SREBP-1 and lipogenic gene expression as well as the levels of liver triglycerides, glycogen, and plasma glucose was partially restored when the fructose diet was supplemented with very high levels of oleate (20% by weight) but not with palmitate, stearate, or linoleate. Fructose in a long term feeding induced the expression of SCD1 and that of other lipogenic genes in the liver of SREBP-1c-/- mice, and a further increase in expression of these genes occurred when the fructose diet was supplemented with oleate. Our observations demonstrated that oleate produced by SCD is necessary for fructose-mediated induction of lipogenic gene expression through SREBP-1c-dependent and -independent mechanisms and suggested that SCD1 gene expression is important in lipid and carbohydrate homeostasis.

Original languageEnglish (US)
Pages (from-to)25164-25171
Number of pages8
JournalJournal of Biological Chemistry
Volume279
Issue number24
DOIs
StatePublished - Jun 11 2004
Externally publishedYes

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Stearoyl-CoA Desaturase
Sterol Regulatory Element Binding Protein 1
Fructose
Gene expression
Oleic Acid
Gene Expression
Genes
Triglycerides
Glucose
Liver Glycogen
Nutrition
Liver
Sterol Regulatory Element Binding Proteins
Diet
Lipids
Plasmas
Stearates
Palmitates
Biosynthesis
Linoleic Acid

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and independent mechanisms. / Miyazaki, Makoto; Dobrzyn, Agnieszka; Man, Weng Chi; Chu, Kiki; Sampath, Harini; Kim, Hyoun Ju; Ntambi, James M.

In: Journal of Biological Chemistry, Vol. 279, No. 24, 11.06.2004, p. 25164-25171.

Research output: Contribution to journalArticle

Miyazaki, Makoto ; Dobrzyn, Agnieszka ; Man, Weng Chi ; Chu, Kiki ; Sampath, Harini ; Kim, Hyoun Ju ; Ntambi, James M. / Stearoyl-CoA desaturase 1 gene expression is necessary for fructose-mediated induction of lipogenic gene expression by sterol regulatory element-binding protein-1c-dependent and independent mechanisms. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 24. pp. 25164-25171.
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abstract = "Stearoyl-CoA desaturase (SCD) synthesizes oleate necessary for the biosynthesis of triglycerides and other lipids. Mice with a targeted disruption of the SCD1 gene are deficient in tissue oleate and have reduced expression of the sterol regulatory element-binding protein (SREBP) and its target genes. The SREBP-1c isoform is a known mediator of insulin action on hepatic gene expression, but its transcriptional effects due to glucose or fructose are still unclear. We found that fructose compared with glucose is a stronger inducer of SREBP-1c and lipogenic gene expression, causing a dramatic increase in hepatic triglyceride levels. However, when fed to the SCD1-/- mice, fructose failed to induce SREBP-1 or lipogenic genes and the triglyceride levels were not increased. Instead fructose feeding caused a decrease in hepatic glycogen and plasma glucose levels. The induction of SREBP-1 and lipogenic gene expression as well as the levels of liver triglycerides, glycogen, and plasma glucose was partially restored when the fructose diet was supplemented with very high levels of oleate (20{\%} by weight) but not with palmitate, stearate, or linoleate. Fructose in a long term feeding induced the expression of SCD1 and that of other lipogenic genes in the liver of SREBP-1c-/- mice, and a further increase in expression of these genes occurred when the fructose diet was supplemented with oleate. Our observations demonstrated that oleate produced by SCD is necessary for fructose-mediated induction of lipogenic gene expression through SREBP-1c-dependent and -independent mechanisms and suggested that SCD1 gene expression is important in lipid and carbohydrate homeostasis.",
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AU - Man, Weng Chi

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AU - Ntambi, James M.

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