Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice

Makoto Miyazaki, Harini Sampath, Xueqing Liu, Matthew T. Flowers, Kiki Chu, Agnieszka Dobrzyn, James M. Ntambi

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Obesity and adiposity greatly increase the risk for secondary conditions such as insulin resistance. Mice deficient in the enzyme stearoyl-CoA desaturase-1 (SCD1) are lean and protected from diet-induced obesity and insulin resistance. In order to determine the effect of SCD1 deficiency on various mouse models of obesity, we introduced a global deletion of the Scd1 gene into leptin-deficient ob/ob mice, leptin-resistant Agouti (Ay/a) mice, and high-fat diet-fed obese (DIO) mice. SCD1 deficiency lowered body weight, adiposity, hepatic lipid accumulation, and hepatic lipogenic gene expression in all three mouse models. However, glucose tolerance, insulin, and leptin sensitivity were improved by SCD1 deficiency only in Ay/a and DIO mice, but not ob/ob mice. These data uncouple the effects of SCD1 deficiency on weight loss from those on insulin sensitivity and suggest a beneficial effect of SCD1 inhibition on insulin sensitivity in obese mice that express a functional leptin gene.

Original languageEnglish (US)
Pages (from-to)818-822
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number4
DOIs
StatePublished - Mar 20 2009
Externally publishedYes

Fingerprint

Stearoyl-CoA Desaturase
Obese Mice
Leptin
Insulin Resistance
Obesity
Insulin
Nutrition
Adiposity
Genes
Diet
Liver
Gene Deletion
High Fat Diet
Gene expression
Weight Loss
Fats
Body Weight
Lipids
Gene Expression
Glucose

Keywords

  • Hepatic lipids
  • High-fat diet
  • Leptin resistance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

Cite this

Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice. / Miyazaki, Makoto; Sampath, Harini; Liu, Xueqing; Flowers, Matthew T.; Chu, Kiki; Dobrzyn, Agnieszka; Ntambi, James M.

In: Biochemical and Biophysical Research Communications, Vol. 380, No. 4, 20.03.2009, p. 818-822.

Research output: Contribution to journalArticle

Miyazaki, Makoto ; Sampath, Harini ; Liu, Xueqing ; Flowers, Matthew T. ; Chu, Kiki ; Dobrzyn, Agnieszka ; Ntambi, James M. / Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice. In: Biochemical and Biophysical Research Communications. 2009 ; Vol. 380, No. 4. pp. 818-822.
@article{902bc7e2f0f145af9e265caedd37b622,
title = "Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice",
abstract = "Obesity and adiposity greatly increase the risk for secondary conditions such as insulin resistance. Mice deficient in the enzyme stearoyl-CoA desaturase-1 (SCD1) are lean and protected from diet-induced obesity and insulin resistance. In order to determine the effect of SCD1 deficiency on various mouse models of obesity, we introduced a global deletion of the Scd1 gene into leptin-deficient ob/ob mice, leptin-resistant Agouti (Ay/a) mice, and high-fat diet-fed obese (DIO) mice. SCD1 deficiency lowered body weight, adiposity, hepatic lipid accumulation, and hepatic lipogenic gene expression in all three mouse models. However, glucose tolerance, insulin, and leptin sensitivity were improved by SCD1 deficiency only in Ay/a and DIO mice, but not ob/ob mice. These data uncouple the effects of SCD1 deficiency on weight loss from those on insulin sensitivity and suggest a beneficial effect of SCD1 inhibition on insulin sensitivity in obese mice that express a functional leptin gene.",
keywords = "Hepatic lipids, High-fat diet, Leptin resistance",
author = "Makoto Miyazaki and Harini Sampath and Xueqing Liu and Flowers, {Matthew T.} and Kiki Chu and Agnieszka Dobrzyn and Ntambi, {James M.}",
year = "2009",
month = "3",
day = "20",
doi = "10.1016/j.bbrc.2009.01.183",
language = "English (US)",
volume = "380",
pages = "818--822",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Stearoyl-CoA desaturase-1 deficiency attenuates obesity and insulin resistance in leptin-resistant obese mice

AU - Miyazaki, Makoto

AU - Sampath, Harini

AU - Liu, Xueqing

AU - Flowers, Matthew T.

AU - Chu, Kiki

AU - Dobrzyn, Agnieszka

AU - Ntambi, James M.

PY - 2009/3/20

Y1 - 2009/3/20

N2 - Obesity and adiposity greatly increase the risk for secondary conditions such as insulin resistance. Mice deficient in the enzyme stearoyl-CoA desaturase-1 (SCD1) are lean and protected from diet-induced obesity and insulin resistance. In order to determine the effect of SCD1 deficiency on various mouse models of obesity, we introduced a global deletion of the Scd1 gene into leptin-deficient ob/ob mice, leptin-resistant Agouti (Ay/a) mice, and high-fat diet-fed obese (DIO) mice. SCD1 deficiency lowered body weight, adiposity, hepatic lipid accumulation, and hepatic lipogenic gene expression in all three mouse models. However, glucose tolerance, insulin, and leptin sensitivity were improved by SCD1 deficiency only in Ay/a and DIO mice, but not ob/ob mice. These data uncouple the effects of SCD1 deficiency on weight loss from those on insulin sensitivity and suggest a beneficial effect of SCD1 inhibition on insulin sensitivity in obese mice that express a functional leptin gene.

AB - Obesity and adiposity greatly increase the risk for secondary conditions such as insulin resistance. Mice deficient in the enzyme stearoyl-CoA desaturase-1 (SCD1) are lean and protected from diet-induced obesity and insulin resistance. In order to determine the effect of SCD1 deficiency on various mouse models of obesity, we introduced a global deletion of the Scd1 gene into leptin-deficient ob/ob mice, leptin-resistant Agouti (Ay/a) mice, and high-fat diet-fed obese (DIO) mice. SCD1 deficiency lowered body weight, adiposity, hepatic lipid accumulation, and hepatic lipogenic gene expression in all three mouse models. However, glucose tolerance, insulin, and leptin sensitivity were improved by SCD1 deficiency only in Ay/a and DIO mice, but not ob/ob mice. These data uncouple the effects of SCD1 deficiency on weight loss from those on insulin sensitivity and suggest a beneficial effect of SCD1 inhibition on insulin sensitivity in obese mice that express a functional leptin gene.

KW - Hepatic lipids

KW - High-fat diet

KW - Leptin resistance

UR - http://www.scopus.com/inward/record.url?scp=60849136007&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60849136007&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.01.183

DO - 10.1016/j.bbrc.2009.01.183

M3 - Article

C2 - 19338759

AN - SCOPUS:60849136007

VL - 380

SP - 818

EP - 822

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -