Steady-state cultures of human skin

Molly Kulesz-Martin, William F. Mclimans

Research output: Contribution to journalArticle

Abstract

Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using automatic-perfusion, thin-layer culture technology (steady state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial–dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalPathobiology
Volume50
Issue number4
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Skin
Langerhans Cells
Melanocytes
Keratinocytes
Osmolar Concentration
Perfusion
Epithelial Cells
Cell Proliferation
Technology
Growth

Keywords

  • Human skin
  • Long-term
  • Optimization
  • Pretransplantation
  • Steady-state culture

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pathology and Forensic Medicine

Cite this

Steady-state cultures of human skin. / Kulesz-Martin, Molly; Mclimans, William F.

In: Pathobiology, Vol. 50, No. 4, 1982, p. 195-206.

Research output: Contribution to journalArticle

Kulesz-Martin, Molly ; Mclimans, William F. / Steady-state cultures of human skin. In: Pathobiology. 1982 ; Vol. 50, No. 4. pp. 195-206.
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