Steady-state cultures of human skin

Molly Kulesz-Martin, W. F. McLimans

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using automatic-perfusion, thin-layer culture technology (steady state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained balanced epithelial-dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
JournalExperimental Cell Biology
Volume50
Issue number4
StatePublished - 1982
Externally publishedYes

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Skin
Langerhans Cells
Melanocytes
Keratinocytes
Osmolar Concentration
Perfusion
Epithelial Cells
Cell Proliferation
Technology
Growth

ASJC Scopus subject areas

  • Cell Biology

Cite this

Steady-state cultures of human skin. / Kulesz-Martin, Molly; McLimans, W. F.

In: Experimental Cell Biology, Vol. 50, No. 4, 1982, p. 195-206.

Research output: Contribution to journalArticle

Kulesz-Martin, M & McLimans, WF 1982, 'Steady-state cultures of human skin', Experimental Cell Biology, vol. 50, no. 4, pp. 195-206.
Kulesz-Martin, Molly ; McLimans, W. F. / Steady-state cultures of human skin. In: Experimental Cell Biology. 1982 ; Vol. 50, No. 4. pp. 195-206.
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