Steady-state cultures of human skin

Molly F. Kulesz-Martin; William F. Mclimans

doi:10.1159/000163147

Steady-state cultures of human skin

Molly F. Kulesz-Martin, William F. Mclimans

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using automatic-perfusion, thin-layer culture technology (steady state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial–dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.

Original language	English (US)
Pages (from-to)	195-206
Number of pages	12
Journal	Pathobiology
Volume	50
Issue number	4
DOIs	https://doi.org/10.1159/000163147
State	Published - 1982
Externally published	Yes

Keywords

Human skin
Long-term
Optimization
Pretransplantation
Steady-state culture

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

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abstract = "Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using automatic-perfusion, thin-layer culture technology (steady state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial–dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.",

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AU - Mclimans, William F.

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AB - Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using automatic-perfusion, thin-layer culture technology (steady state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial–dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.

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KW - Long-term

KW - Optimization

KW - Pretransplantation

KW - Steady-state culture

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Steady-state cultures of human skin

Abstract

Keywords

ASJC Scopus subject areas

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