Steady-state cultures of human skin

Molly F. Kulesz-Martin, William F. Mclimans

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Pretransplantation cultivation of adult human skin has been optimized for rapid and prolonged outgrowth of epidermal cells from tissue explants using automatic-perfusion, thin-layer culture technology (steady state). This system fostered growth of autologous mesenchymal elements via critical control of the culture environment. The resulting cellular outgrowth maintained a balanced epithelial–dermal relationship, contained keratinocytes as well as minority epidermal cells, melanocytes and possibly Langerhans cells. Critical control of culture pH and osmolarity was found to enhance epithelial cell proliferation.

Original languageEnglish (US)
Pages (from-to)195-206
Number of pages12
Issue number4
StatePublished - Jan 1 1982
Externally publishedYes


  • Human skin
  • Long-term
  • Optimization
  • Pretransplantation
  • Steady-state culture

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology


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