STAT3 in epithelial cells regulates inflammation and tumor progression to malignant state in colon

Andrew V. Nguyen, Yuan Yuan Wu, Qiang Liu, Donghai Wang, Stephanie Nguyen, Ricky Loh, Joey Pang, Kenneth Friedman, Amos Orlofsky, Leonard Augenlicht, Jeffrey W. Pollard, Elaine Lin

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Chronic inflammation is an important risk factor for the development of colorectal cancer; however, the mechanism of tumorigenesis especially tumor progression to malignancy in the inflamed colon is still unclear. Our study shows that epithelial signal transducer and activator of transcription 3 (STAT3), persistently activated in inflamed colon, is not required for inflammation-induced epithelial overproliferation and the development of early-stage tumors; however, it is essential for tumor progression to advanced malignancy. We found that one of the mechanisms that epithelial STAT3 regulates in tumor progression might be to modify leukocytic infiltration in the large intestine. Activation of epithelial STAT3 promotes the infiltration of the CD8+ lymphocyte population but inhibits the recruitment of regulatory T (Treg) lymphocytes. The loss of Stat3 in epithelial cells promoted the expression of cytokines/chemokines including CCL19, CCL28, and RANTES, which are known to be able to recruit Treg lymphocytes. Linked to these changes was the pathway mediated by sphingosine 1-phosphate receptor 1 and sphingosine 1-phosphate kinases, which is activated in colonic epithelial cells in inflamed colon with functional STAT3 but not in epithelial cells deleted of STAT3. Our data suggest that epithelial STAT3 plays a critical role in inflammation-induced tumor progression through regulation of leukocytic recruitment especially the infiltration of Treg cells in the large intestine.

Original languageEnglish (US)
Pages (from-to)998-1008
Number of pages11
JournalNeoplasia (United States)
Volume15
Issue number9
DOIs
StatePublished - Sep 2013
Externally publishedYes

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STAT3 Transcription Factor
Colon
Epithelial Cells
Inflammation
Neoplasms
Large Intestine
Regulatory T-Lymphocytes
Chemokine CCL19
Lysosphingolipid Receptors
Lymphocytes
Chemokine CCL5
Colorectal Neoplasms
Carcinogenesis
Phosphotransferases
Cytokines
Population

ASJC Scopus subject areas

  • Cancer Research

Cite this

Nguyen, A. V., Wu, Y. Y., Liu, Q., Wang, D., Nguyen, S., Loh, R., ... Lin, E. (2013). STAT3 in epithelial cells regulates inflammation and tumor progression to malignant state in colon. Neoplasia (United States), 15(9), 998-1008. https://doi.org/10.1593/neo.13952

STAT3 in epithelial cells regulates inflammation and tumor progression to malignant state in colon. / Nguyen, Andrew V.; Wu, Yuan Yuan; Liu, Qiang; Wang, Donghai; Nguyen, Stephanie; Loh, Ricky; Pang, Joey; Friedman, Kenneth; Orlofsky, Amos; Augenlicht, Leonard; Pollard, Jeffrey W.; Lin, Elaine.

In: Neoplasia (United States), Vol. 15, No. 9, 09.2013, p. 998-1008.

Research output: Contribution to journalArticle

Nguyen, AV, Wu, YY, Liu, Q, Wang, D, Nguyen, S, Loh, R, Pang, J, Friedman, K, Orlofsky, A, Augenlicht, L, Pollard, JW & Lin, E 2013, 'STAT3 in epithelial cells regulates inflammation and tumor progression to malignant state in colon', Neoplasia (United States), vol. 15, no. 9, pp. 998-1008. https://doi.org/10.1593/neo.13952
Nguyen, Andrew V. ; Wu, Yuan Yuan ; Liu, Qiang ; Wang, Donghai ; Nguyen, Stephanie ; Loh, Ricky ; Pang, Joey ; Friedman, Kenneth ; Orlofsky, Amos ; Augenlicht, Leonard ; Pollard, Jeffrey W. ; Lin, Elaine. / STAT3 in epithelial cells regulates inflammation and tumor progression to malignant state in colon. In: Neoplasia (United States). 2013 ; Vol. 15, No. 9. pp. 998-1008.
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