STAT3 and SMAD1 signaling in medaka embryonic stem-like cells and blastula embryos

T. U. Wagner, M. Kraeussling, Lev Fedorov, C. Reiss, B. Kneitz, M. Schartl

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these systems. At the same time, BMP signaling via smad1 was shown to be able to counteract STAT3 signaling in murine ES-cell cultures, while it influences differentiation in multifaceted ways in other cellular contexts. Hence, the question arises whether the signaling situation found in mice or primates and human ES-cells represent the rule or the exception. With this study, we want to contribute an answer to this question from an evolutionary perspective. Therefore, we analyzed the expression and activation status of the Medaka (Oryzias latipes) STAT3 and SMAD1 in Medaka ES-cell-like cultures and their in vivo counterpart, the Medaka blastula embryo. While SMAD signaling is active in the culture system as well as in blastula embryos, our results indicate that STAT3 is inactive and can thus not be involved in pluripotency control of blastula cells or their derived pluripotent in vitro counterparts. These results suggest that the signaling pathways active in the mouse ES-cell culture system represent the exception, while inactivity of STAT3 is apparently the rule in vertebrate ES-cell cultures.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalStem Cells and Development
Volume18
Issue number1
DOIs
StatePublished - Jan 1 2009
Externally publishedYes

Fingerprint

Oryzias
Blastula
STAT3 Transcription Factor
Embryonic Stem Cells
Embryonic Structures
Cell Culture Techniques
Primates
Transcriptional Activation
Haplorhini
Vertebrates

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology
  • Medicine(all)

Cite this

STAT3 and SMAD1 signaling in medaka embryonic stem-like cells and blastula embryos. / Wagner, T. U.; Kraeussling, M.; Fedorov, Lev; Reiss, C.; Kneitz, B.; Schartl, M.

In: Stem Cells and Development, Vol. 18, No. 1, 01.01.2009, p. 151-160.

Research output: Contribution to journalArticle

Wagner, T. U. ; Kraeussling, M. ; Fedorov, Lev ; Reiss, C. ; Kneitz, B. ; Schartl, M. / STAT3 and SMAD1 signaling in medaka embryonic stem-like cells and blastula embryos. In: Stem Cells and Development. 2009 ; Vol. 18, No. 1. pp. 151-160.
@article{e24e84bb0a1841f78ce67fde33a59943,
title = "STAT3 and SMAD1 signaling in medaka embryonic stem-like cells and blastula embryos",
abstract = "The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these systems. At the same time, BMP signaling via smad1 was shown to be able to counteract STAT3 signaling in murine ES-cell cultures, while it influences differentiation in multifaceted ways in other cellular contexts. Hence, the question arises whether the signaling situation found in mice or primates and human ES-cells represent the rule or the exception. With this study, we want to contribute an answer to this question from an evolutionary perspective. Therefore, we analyzed the expression and activation status of the Medaka (Oryzias latipes) STAT3 and SMAD1 in Medaka ES-cell-like cultures and their in vivo counterpart, the Medaka blastula embryo. While SMAD signaling is active in the culture system as well as in blastula embryos, our results indicate that STAT3 is inactive and can thus not be involved in pluripotency control of blastula cells or their derived pluripotent in vitro counterparts. These results suggest that the signaling pathways active in the mouse ES-cell culture system represent the exception, while inactivity of STAT3 is apparently the rule in vertebrate ES-cell cultures.",
author = "Wagner, {T. U.} and M. Kraeussling and Lev Fedorov and C. Reiss and B. Kneitz and M. Schartl",
year = "2009",
month = "1",
day = "1",
doi = "10.1089/scd.2007.0262",
language = "English (US)",
volume = "18",
pages = "151--160",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary Ann Liebert Inc.",
number = "1",

}

TY - JOUR

T1 - STAT3 and SMAD1 signaling in medaka embryonic stem-like cells and blastula embryos

AU - Wagner, T. U.

AU - Kraeussling, M.

AU - Fedorov, Lev

AU - Reiss, C.

AU - Kneitz, B.

AU - Schartl, M.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these systems. At the same time, BMP signaling via smad1 was shown to be able to counteract STAT3 signaling in murine ES-cell cultures, while it influences differentiation in multifaceted ways in other cellular contexts. Hence, the question arises whether the signaling situation found in mice or primates and human ES-cells represent the rule or the exception. With this study, we want to contribute an answer to this question from an evolutionary perspective. Therefore, we analyzed the expression and activation status of the Medaka (Oryzias latipes) STAT3 and SMAD1 in Medaka ES-cell-like cultures and their in vivo counterpart, the Medaka blastula embryo. While SMAD signaling is active in the culture system as well as in blastula embryos, our results indicate that STAT3 is inactive and can thus not be involved in pluripotency control of blastula cells or their derived pluripotent in vitro counterparts. These results suggest that the signaling pathways active in the mouse ES-cell culture system represent the exception, while inactivity of STAT3 is apparently the rule in vertebrate ES-cell cultures.

AB - The activation and transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is essential for maintaining mouse embryonic stem (ES) cell cultures in an undifferentiated state. However, reports from human and monkey ES-cell culture suggest that STAT3 is dispensable for pluripotency in these systems. At the same time, BMP signaling via smad1 was shown to be able to counteract STAT3 signaling in murine ES-cell cultures, while it influences differentiation in multifaceted ways in other cellular contexts. Hence, the question arises whether the signaling situation found in mice or primates and human ES-cells represent the rule or the exception. With this study, we want to contribute an answer to this question from an evolutionary perspective. Therefore, we analyzed the expression and activation status of the Medaka (Oryzias latipes) STAT3 and SMAD1 in Medaka ES-cell-like cultures and their in vivo counterpart, the Medaka blastula embryo. While SMAD signaling is active in the culture system as well as in blastula embryos, our results indicate that STAT3 is inactive and can thus not be involved in pluripotency control of blastula cells or their derived pluripotent in vitro counterparts. These results suggest that the signaling pathways active in the mouse ES-cell culture system represent the exception, while inactivity of STAT3 is apparently the rule in vertebrate ES-cell cultures.

UR - http://www.scopus.com/inward/record.url?scp=60149103801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=60149103801&partnerID=8YFLogxK

U2 - 10.1089/scd.2007.0262

DO - 10.1089/scd.2007.0262

M3 - Article

C2 - 18554090

AN - SCOPUS:60149103801

VL - 18

SP - 151

EP - 160

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 1

ER -