Staphylococcus-induced urolithiasis in estrogen-treated ovariectomized nude mice

Lona K. Gibbs, Debra L. Hickman, Anne Lewis, Lois Colgin

    Research output: Contribution to journalArticle

    6 Citations (Scopus)

    Abstract

    Nine of 24 ovariectomized nude mice developed ulcerative skin lesions 28 d after implantation with human breast cancer cells and slow-release estrogen pellets. Aerobic culture of samples from the skin lesions yielded Staphylococcus intermedius. By day 45 postimplantation, all mice displayed ulcerative skin lesions, and 5 mice exhibited hunched posture, listless behavior, cyanosis, anorexia, and dehydration. This subset was euthanized but not necropsied. When additional animals became moribund, the investigator elected to end the study at day 51. At necropsy, all 20 but 1 had cystitis and urolithiasis, characterized by relatively large struvite stones and crystals that had filled the urinary bladders of the research animals and caused severe thickening of the bladder walls. Gram-positive cocci were observed microscopically in both the kidneys and bladders of the necropsied animals. Spontaneous urolithiasis has not previously been documented to occur in association with infection of female nude mice by S. intermedius.

    Original languageEnglish (US)
    Pages (from-to)61-65
    Number of pages5
    JournalJournal of the American Association for Laboratory Animal Science
    Volume46
    Issue number4
    StatePublished - Jul 2007

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    Staphylococcus
    estrogens
    skin lesions
    bladder
    Staphylococcus intermedius
    mice
    magnesium ammonium phosphate
    cystitis
    dehydration (animal physiology)
    Coccus
    posture
    anorexia
    breast neoplasms
    laboratory animals
    crystals
    pellets
    necropsy
    animals
    kidneys
    urolithiasis

    ASJC Scopus subject areas

    • Animal Science and Zoology

    Cite this

    Staphylococcus-induced urolithiasis in estrogen-treated ovariectomized nude mice. / Gibbs, Lona K.; Hickman, Debra L.; Lewis, Anne; Colgin, Lois.

    In: Journal of the American Association for Laboratory Animal Science, Vol. 46, No. 4, 07.2007, p. 61-65.

    Research output: Contribution to journalArticle

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