Standard-Dose Intradermal Influenza Vaccine Elicits Cellular Immune Responses Similar to Those of Intramuscular Vaccine in Men With and Those Without HIV Infection

Samuel Amoah, Margarita Mishina, Prabda Praphasiri, Weiping Cao, Jin Hyang Kim, Justine S. Liepkalns, Zhu Guo, Paul J. Carney, Jessie C. Chang, Stefan Fernandez, Shikha Garg, Lauren Beacham, Timothy H. Holtz, Marcel Curlin, Fatimah Dawood, Sonja J. Olsen, Shivaprakash Gangappa, James Stevens, Suryaprakash Sambhara

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Human immunodeficiency virus (HIV)-infected persons are at a higher risk of severe influenza. Although we have shown that a standard-dose intradermal influenza vaccine versus a standard-dose intramuscular influenza vaccine does not result in differences in hemagglutination-inhibition titers in this population, a comprehensive examination of cell-mediated immune responses remains lacking. METHODS: Serological, antigen-specific B-cell, and interleukin 2-, interferon γ-, and tumor necrosis factor α-secreting T-cell responses were assessed in 79 HIV-infected men and 79 HIV-uninfected men. RESULTS: The route of vaccination did not affect the immunoglobulin A and immunoglobulin G (IgG) plasmablast or memory B-cell response, although these were severely impaired in the group with a CD4+ T-cell count of <200 cells/μL. The frequencies of IgG memory B cells measured on day 28 after vaccination were highest in the HIV-uninfected group, followed by the group with a CD4+ T-cell count of ≥200 cells/μL and the group with a CD4+ T-cell count of <200 cells/μL. The route of vaccination did not affect the CD4+ or CD8+ T-cell responses measured at various times after vaccination. CONCLUSIONS: The route of vaccination had no effect on antibody responses, antibody avidity, T-cell responses, or B-cell responses in HIV-infected or HIV-uninfected subjects. With the serological and cellular immune responses to influenza vaccination being impaired in HIV-infected individuals with a CD4+ T-cell count of <200 cells/μL, passive immunization strategies need to be explored to protect this population. CLINICAL TRIALS REGISTRATION: NCT01538940.

Original languageEnglish (US)
Pages (from-to)743-751
Number of pages9
JournalThe Journal of infectious diseases
Volume220
Issue number5
DOIs
StatePublished - Jul 31 2019

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Influenza Vaccines
Virus Diseases
Cellular Immunity
Vaccines
HIV
Vaccination
T-Lymphocytes
CD4 Lymphocyte Count
B-Lymphocytes
Human Influenza
Immunoglobulin G
Antibody Affinity
Passive Immunization
Hemagglutination
Immunoglobulin A
Interferons
Population
Antibody Formation
Interleukin-2
Tumor Necrosis Factor-alpha

Keywords

  • cell-mediated
  • HIV
  • Influenza
  • intradermal
  • intramuscular
  • vaccination

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Standard-Dose Intradermal Influenza Vaccine Elicits Cellular Immune Responses Similar to Those of Intramuscular Vaccine in Men With and Those Without HIV Infection. / Amoah, Samuel; Mishina, Margarita; Praphasiri, Prabda; Cao, Weiping; Kim, Jin Hyang; Liepkalns, Justine S.; Guo, Zhu; Carney, Paul J.; Chang, Jessie C.; Fernandez, Stefan; Garg, Shikha; Beacham, Lauren; Holtz, Timothy H.; Curlin, Marcel; Dawood, Fatimah; Olsen, Sonja J.; Gangappa, Shivaprakash; Stevens, James; Sambhara, Suryaprakash.

In: The Journal of infectious diseases, Vol. 220, No. 5, 31.07.2019, p. 743-751.

Research output: Contribution to journalArticle

Amoah, S, Mishina, M, Praphasiri, P, Cao, W, Kim, JH, Liepkalns, JS, Guo, Z, Carney, PJ, Chang, JC, Fernandez, S, Garg, S, Beacham, L, Holtz, TH, Curlin, M, Dawood, F, Olsen, SJ, Gangappa, S, Stevens, J & Sambhara, S 2019, 'Standard-Dose Intradermal Influenza Vaccine Elicits Cellular Immune Responses Similar to Those of Intramuscular Vaccine in Men With and Those Without HIV Infection', The Journal of infectious diseases, vol. 220, no. 5, pp. 743-751. https://doi.org/10.1093/infdis/jiz205
Amoah, Samuel ; Mishina, Margarita ; Praphasiri, Prabda ; Cao, Weiping ; Kim, Jin Hyang ; Liepkalns, Justine S. ; Guo, Zhu ; Carney, Paul J. ; Chang, Jessie C. ; Fernandez, Stefan ; Garg, Shikha ; Beacham, Lauren ; Holtz, Timothy H. ; Curlin, Marcel ; Dawood, Fatimah ; Olsen, Sonja J. ; Gangappa, Shivaprakash ; Stevens, James ; Sambhara, Suryaprakash. / Standard-Dose Intradermal Influenza Vaccine Elicits Cellular Immune Responses Similar to Those of Intramuscular Vaccine in Men With and Those Without HIV Infection. In: The Journal of infectious diseases. 2019 ; Vol. 220, No. 5. pp. 743-751.
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abstract = "BACKGROUND: Human immunodeficiency virus (HIV)-infected persons are at a higher risk of severe influenza. Although we have shown that a standard-dose intradermal influenza vaccine versus a standard-dose intramuscular influenza vaccine does not result in differences in hemagglutination-inhibition titers in this population, a comprehensive examination of cell-mediated immune responses remains lacking. METHODS: Serological, antigen-specific B-cell, and interleukin 2-, interferon γ-, and tumor necrosis factor α-secreting T-cell responses were assessed in 79 HIV-infected men and 79 HIV-uninfected men. RESULTS: The route of vaccination did not affect the immunoglobulin A and immunoglobulin G (IgG) plasmablast or memory B-cell response, although these were severely impaired in the group with a CD4+ T-cell count of <200 cells/μL. The frequencies of IgG memory B cells measured on day 28 after vaccination were highest in the HIV-uninfected group, followed by the group with a CD4+ T-cell count of ≥200 cells/μL and the group with a CD4+ T-cell count of <200 cells/μL. The route of vaccination did not affect the CD4+ or CD8+ T-cell responses measured at various times after vaccination. CONCLUSIONS: The route of vaccination had no effect on antibody responses, antibody avidity, T-cell responses, or B-cell responses in HIV-infected or HIV-uninfected subjects. With the serological and cellular immune responses to influenza vaccination being impaired in HIV-infected individuals with a CD4+ T-cell count of <200 cells/μL, passive immunization strategies need to be explored to protect this population. CLINICAL TRIALS REGISTRATION: NCT01538940.",
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T1 - Standard-Dose Intradermal Influenza Vaccine Elicits Cellular Immune Responses Similar to Those of Intramuscular Vaccine in Men With and Those Without HIV Infection

AU - Amoah, Samuel

AU - Mishina, Margarita

AU - Praphasiri, Prabda

AU - Cao, Weiping

AU - Kim, Jin Hyang

AU - Liepkalns, Justine S.

AU - Guo, Zhu

AU - Carney, Paul J.

AU - Chang, Jessie C.

AU - Fernandez, Stefan

AU - Garg, Shikha

AU - Beacham, Lauren

AU - Holtz, Timothy H.

AU - Curlin, Marcel

AU - Dawood, Fatimah

AU - Olsen, Sonja J.

AU - Gangappa, Shivaprakash

AU - Stevens, James

AU - Sambhara, Suryaprakash

PY - 2019/7/31

Y1 - 2019/7/31

N2 - BACKGROUND: Human immunodeficiency virus (HIV)-infected persons are at a higher risk of severe influenza. Although we have shown that a standard-dose intradermal influenza vaccine versus a standard-dose intramuscular influenza vaccine does not result in differences in hemagglutination-inhibition titers in this population, a comprehensive examination of cell-mediated immune responses remains lacking. METHODS: Serological, antigen-specific B-cell, and interleukin 2-, interferon γ-, and tumor necrosis factor α-secreting T-cell responses were assessed in 79 HIV-infected men and 79 HIV-uninfected men. RESULTS: The route of vaccination did not affect the immunoglobulin A and immunoglobulin G (IgG) plasmablast or memory B-cell response, although these were severely impaired in the group with a CD4+ T-cell count of <200 cells/μL. The frequencies of IgG memory B cells measured on day 28 after vaccination were highest in the HIV-uninfected group, followed by the group with a CD4+ T-cell count of ≥200 cells/μL and the group with a CD4+ T-cell count of <200 cells/μL. The route of vaccination did not affect the CD4+ or CD8+ T-cell responses measured at various times after vaccination. CONCLUSIONS: The route of vaccination had no effect on antibody responses, antibody avidity, T-cell responses, or B-cell responses in HIV-infected or HIV-uninfected subjects. With the serological and cellular immune responses to influenza vaccination being impaired in HIV-infected individuals with a CD4+ T-cell count of <200 cells/μL, passive immunization strategies need to be explored to protect this population. CLINICAL TRIALS REGISTRATION: NCT01538940.

AB - BACKGROUND: Human immunodeficiency virus (HIV)-infected persons are at a higher risk of severe influenza. Although we have shown that a standard-dose intradermal influenza vaccine versus a standard-dose intramuscular influenza vaccine does not result in differences in hemagglutination-inhibition titers in this population, a comprehensive examination of cell-mediated immune responses remains lacking. METHODS: Serological, antigen-specific B-cell, and interleukin 2-, interferon γ-, and tumor necrosis factor α-secreting T-cell responses were assessed in 79 HIV-infected men and 79 HIV-uninfected men. RESULTS: The route of vaccination did not affect the immunoglobulin A and immunoglobulin G (IgG) plasmablast or memory B-cell response, although these were severely impaired in the group with a CD4+ T-cell count of <200 cells/μL. The frequencies of IgG memory B cells measured on day 28 after vaccination were highest in the HIV-uninfected group, followed by the group with a CD4+ T-cell count of ≥200 cells/μL and the group with a CD4+ T-cell count of <200 cells/μL. The route of vaccination did not affect the CD4+ or CD8+ T-cell responses measured at various times after vaccination. CONCLUSIONS: The route of vaccination had no effect on antibody responses, antibody avidity, T-cell responses, or B-cell responses in HIV-infected or HIV-uninfected subjects. With the serological and cellular immune responses to influenza vaccination being impaired in HIV-infected individuals with a CD4+ T-cell count of <200 cells/μL, passive immunization strategies need to be explored to protect this population. CLINICAL TRIALS REGISTRATION: NCT01538940.

KW - cell-mediated

KW - HIV

KW - Influenza

KW - intradermal

KW - intramuscular

KW - vaccination

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