Stage-specific modulation of antimüllerian hormone promotes primate follicular development and oocyte maturation in the matrix-free three-dimensional culture

Jing Xu, Maralee S. Lawson, Shoukhrat Mitalipov, Byung Park, Fuhua Xu

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To study whether follicular growth and oocyte maturation can be improved by antimüllerian hormone (AMH) modulation at specific stages of follicular development. Design: Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting: National primate research center. Animal(s): Adult, female rhesus macaques (Macaca mulatta). Intervention(s): None. Main Outcome Measure(s): Follicle survival, growth, steroid and paracrine factor production, and oocyte competence were evaluated. Follicles were assessed for expression of genes that are critical for gonadotropin signaling, cumulus cell glycolysis, and oocyte quality. Result(s): Primary follicles formed “organoids” and developed to the antral stage in group culture. AMH exposure during the preantral stage increased organoid diameters. Oocytes from the AMH-treated organoids had greater diameters and matured to the metaphase II (MII) stage. Secondary follicles developed to the antral stage during individual culture. The AMH exposure during the preantral stage and AMH antibody treatment during the antral stage increased follicle diameters, vascular endothelial growth factor and follistatin production, differentiation factor 9 expression, and oocyte diameters. The MII oocytes from the AMH-modulated group developed to the morula stage after IVF, with one to the blastocyst stage. Conclusion(s): AMH supplementation at the preantral stage and depletion at the antral stage enhanced primate follicular development and oocyte competence in vitro. The improved embryonic development supports in vitro follicle maturation as a potential approach for fertility preservation.

Original languageEnglish (US)
Pages (from-to)1162-1172
Number of pages11
JournalFertility and Sterility
Volume110
Issue number6
DOIs
StatePublished - Nov 1 2018

Fingerprint

Primates
Oocytes
Hormones
Organoids
Metaphase
Macaca mulatta
Mental Competency
Follistatin
Fertility Preservation
Cumulus Cells
Morula
Antibodies
Blastocyst
Glycolysis
Growth
Gonadotropins
Vascular Endothelial Growth Factor A
Embryonic Development
Steroids
Outcome Assessment (Health Care)

Keywords

  • Antimüllerian hormone
  • fertility preservation
  • follicle culture
  • matrix-free
  • oocyte maturation

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology

Cite this

@article{1550f88d17ca4cc1a06eaf21d02c7c67,
title = "Stage-specific modulation of antim{\"u}llerian hormone promotes primate follicular development and oocyte maturation in the matrix-free three-dimensional culture",
abstract = "Objective: To study whether follicular growth and oocyte maturation can be improved by antim{\"u}llerian hormone (AMH) modulation at specific stages of follicular development. Design: Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting: National primate research center. Animal(s): Adult, female rhesus macaques (Macaca mulatta). Intervention(s): None. Main Outcome Measure(s): Follicle survival, growth, steroid and paracrine factor production, and oocyte competence were evaluated. Follicles were assessed for expression of genes that are critical for gonadotropin signaling, cumulus cell glycolysis, and oocyte quality. Result(s): Primary follicles formed “organoids” and developed to the antral stage in group culture. AMH exposure during the preantral stage increased organoid diameters. Oocytes from the AMH-treated organoids had greater diameters and matured to the metaphase II (MII) stage. Secondary follicles developed to the antral stage during individual culture. The AMH exposure during the preantral stage and AMH antibody treatment during the antral stage increased follicle diameters, vascular endothelial growth factor and follistatin production, differentiation factor 9 expression, and oocyte diameters. The MII oocytes from the AMH-modulated group developed to the morula stage after IVF, with one to the blastocyst stage. Conclusion(s): AMH supplementation at the preantral stage and depletion at the antral stage enhanced primate follicular development and oocyte competence in vitro. The improved embryonic development supports in vitro follicle maturation as a potential approach for fertility preservation.",
keywords = "Antim{\"u}llerian hormone, fertility preservation, follicle culture, matrix-free, oocyte maturation",
author = "Jing Xu and Lawson, {Maralee S.} and Shoukhrat Mitalipov and Byung Park and Fuhua Xu",
year = "2018",
month = "11",
day = "1",
doi = "10.1016/j.fertnstert.2018.07.006",
language = "English (US)",
volume = "110",
pages = "1162--1172",
journal = "Fertility and Sterility",
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TY - JOUR

T1 - Stage-specific modulation of antimüllerian hormone promotes primate follicular development and oocyte maturation in the matrix-free three-dimensional culture

AU - Xu, Jing

AU - Lawson, Maralee S.

AU - Mitalipov, Shoukhrat

AU - Park, Byung

AU - Xu, Fuhua

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Objective: To study whether follicular growth and oocyte maturation can be improved by antimüllerian hormone (AMH) modulation at specific stages of follicular development. Design: Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting: National primate research center. Animal(s): Adult, female rhesus macaques (Macaca mulatta). Intervention(s): None. Main Outcome Measure(s): Follicle survival, growth, steroid and paracrine factor production, and oocyte competence were evaluated. Follicles were assessed for expression of genes that are critical for gonadotropin signaling, cumulus cell glycolysis, and oocyte quality. Result(s): Primary follicles formed “organoids” and developed to the antral stage in group culture. AMH exposure during the preantral stage increased organoid diameters. Oocytes from the AMH-treated organoids had greater diameters and matured to the metaphase II (MII) stage. Secondary follicles developed to the antral stage during individual culture. The AMH exposure during the preantral stage and AMH antibody treatment during the antral stage increased follicle diameters, vascular endothelial growth factor and follistatin production, differentiation factor 9 expression, and oocyte diameters. The MII oocytes from the AMH-modulated group developed to the morula stage after IVF, with one to the blastocyst stage. Conclusion(s): AMH supplementation at the preantral stage and depletion at the antral stage enhanced primate follicular development and oocyte competence in vitro. The improved embryonic development supports in vitro follicle maturation as a potential approach for fertility preservation.

AB - Objective: To study whether follicular growth and oocyte maturation can be improved by antimüllerian hormone (AMH) modulation at specific stages of follicular development. Design: Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage. Setting: National primate research center. Animal(s): Adult, female rhesus macaques (Macaca mulatta). Intervention(s): None. Main Outcome Measure(s): Follicle survival, growth, steroid and paracrine factor production, and oocyte competence were evaluated. Follicles were assessed for expression of genes that are critical for gonadotropin signaling, cumulus cell glycolysis, and oocyte quality. Result(s): Primary follicles formed “organoids” and developed to the antral stage in group culture. AMH exposure during the preantral stage increased organoid diameters. Oocytes from the AMH-treated organoids had greater diameters and matured to the metaphase II (MII) stage. Secondary follicles developed to the antral stage during individual culture. The AMH exposure during the preantral stage and AMH antibody treatment during the antral stage increased follicle diameters, vascular endothelial growth factor and follistatin production, differentiation factor 9 expression, and oocyte diameters. The MII oocytes from the AMH-modulated group developed to the morula stage after IVF, with one to the blastocyst stage. Conclusion(s): AMH supplementation at the preantral stage and depletion at the antral stage enhanced primate follicular development and oocyte competence in vitro. The improved embryonic development supports in vitro follicle maturation as a potential approach for fertility preservation.

KW - Antimüllerian hormone

KW - fertility preservation

KW - follicle culture

KW - matrix-free

KW - oocyte maturation

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U2 - 10.1016/j.fertnstert.2018.07.006

DO - 10.1016/j.fertnstert.2018.07.006

M3 - Article

VL - 110

SP - 1162

EP - 1172

JO - Fertility and Sterility

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SN - 0015-0282

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