Stabilization of hypoxia-inducible factor-1α in buffer containing cobalt chloride for Western blot analysis

Sathyanarayanan Srinivasan, Jeff F. Dunn

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to stabilize HIF-1α protein during sample preparation for Western blot analysis due to its rapid degradation in the presence of oxygen. Enzyme inhibitor cocktails can be complex and expensive. We present a protease inhibitor-free buffer, containing cobalt chloride, which is effective at stabilizing HIF-1α, while being inexpensive, straightforward, and convenient, and has potential for widespread application.

Original languageEnglish (US)
Pages (from-to)120-122
Number of pages3
JournalAnalytical Biochemistry
Volume416
Issue number1
DOIs
StatePublished - Sep 1 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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