TY - JOUR
T1 - Stabilization of hypoxia-inducible factor-1α in buffer containing cobalt chloride for Western blot analysis
AU - Srinivasan, Sathyanarayanan
AU - Dunn, Jeff F.
N1 - Funding Information:
We thank Dr. Faton Agani (Department of Anatomy, Case Western Reserve University, Ohio, USA) for the HIF-1α antibody. This work was partially supported by an NIH RO1 ( EB002085 ), and the Canadian Institutes of Health Research (CIHR) FIN 79260 and Alberta Innovates-Health Solutions to J.F.D.
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to stabilize HIF-1α protein during sample preparation for Western blot analysis due to its rapid degradation in the presence of oxygen. Enzyme inhibitor cocktails can be complex and expensive. We present a protease inhibitor-free buffer, containing cobalt chloride, which is effective at stabilizing HIF-1α, while being inexpensive, straightforward, and convenient, and has potential for widespread application.
AB - Hypoxia-inducible factor-1α (HIF-1α) is a widely studied protein with significant biomedical impact. Care is needed to stabilize HIF-1α protein during sample preparation for Western blot analysis due to its rapid degradation in the presence of oxygen. Enzyme inhibitor cocktails can be complex and expensive. We present a protease inhibitor-free buffer, containing cobalt chloride, which is effective at stabilizing HIF-1α, while being inexpensive, straightforward, and convenient, and has potential for widespread application.
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U2 - 10.1016/j.ab.2011.04.037
DO - 10.1016/j.ab.2011.04.037
M3 - Article
C2 - 21601556
AN - SCOPUS:79959748491
SN - 0003-2697
VL - 416
SP - 120
EP - 122
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -