Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades

Yiling Lu, Qinghua Yu, Jue Hui Liu, Jinyi Zhang, Hongwei Wang, Dimpy Koul, John S. McMurray, Xianjun Fang, W. K Alfred Yung, Kathy A. Siminovitch, Gordon Mills

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Src family protein-tyrosine kinases, which play an important role in signal integration, have been implicated in tumorigenesis in multiple lineages, including breast cancer. We demonstrate, herein, that Src kinases regulate the phosphatidylinositol 3-kinase (PI3K) signaling cascade via altering the function of the PTEN tumor suppressor. Overexpression of activated Src protein-tyrosine kinases in PTEN-deficient breast cancer cells does not alter AKT phosphorylation, an indicator of signal transduction through the PI3K pathway. However, in the presence of functional PTEN, Src reverses the activity of PTEN, resulting in an increase in AKT phosphorylation. Activated Src reduces the ability of PTEN to dephosphorylate phosphatidylinositols in micelles and promotes AKT translocation to cellular plasma membranes but does not alter PTEN activity toward water-soluble phosphatidylinositols. Thus, Src may alter the capacity of the PTEN C2 domain to bind cellular membranes rather than directly interfering with PTEN enzymatic activity. Tyrosine phosphorylation of PTEN is increased in breast cancer cells treated with pervanadate, suggesting that PTEN contains sites for tyrosine phosphorylation. Src kinase inhibitors markedly decreased pervanadate-mediated tyrosine phosphorylation of PTEN. Further, expression of activated Src results in marked tyrosine phosphorylation of PTEN. SHP-1, a SH2 domain-containing protein-tyrosine phosphatase, selectively binds and dephosphorylates PTEN in Src transfected cells. Both Src inhibitors and SHP-1 overexpression reverse Src-induced loss of PTEN function. Coexpression of PTEN with activated Src reduces the stability of PTEN. Taken together, the data indicate that activated Src inhibits PTEN function leading to alterations in signaling through the PI3K/AKT pathway.

Original languageEnglish (US)
Pages (from-to)40057-40066
Number of pages10
JournalJournal of Biological Chemistry
Volume278
Issue number41
DOIs
StatePublished - Oct 10 2003
Externally publishedYes

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Phosphatidylinositol 3-Kinase
Phosphorylation
src-Family Kinases
Protein-Tyrosine Kinases
Tyrosine
Breast Neoplasms
Phosphatidylinositols
SH2 Domain-Containing Protein Tyrosine Phosphatases
Cells
Signal transduction
Micelles
Cell membranes
Tumors
Signal Transduction
Carcinogenesis
Cell Membrane
Membranes
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades. / Lu, Yiling; Yu, Qinghua; Liu, Jue Hui; Zhang, Jinyi; Wang, Hongwei; Koul, Dimpy; McMurray, John S.; Fang, Xianjun; Yung, W. K Alfred; Siminovitch, Kathy A.; Mills, Gordon.

In: Journal of Biological Chemistry, Vol. 278, No. 41, 10.10.2003, p. 40057-40066.

Research output: Contribution to journalArticle

Lu, Y, Yu, Q, Liu, JH, Zhang, J, Wang, H, Koul, D, McMurray, JS, Fang, X, Yung, WKA, Siminovitch, KA & Mills, G 2003, 'Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades', Journal of Biological Chemistry, vol. 278, no. 41, pp. 40057-40066. https://doi.org/10.1074/jbc.M303621200
Lu, Yiling ; Yu, Qinghua ; Liu, Jue Hui ; Zhang, Jinyi ; Wang, Hongwei ; Koul, Dimpy ; McMurray, John S. ; Fang, Xianjun ; Yung, W. K Alfred ; Siminovitch, Kathy A. ; Mills, Gordon. / Src family protein-tyrosine kinases alter the function of PTEN to regulate phosphatidylinositol 3-kinase/AKT cascades. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 41. pp. 40057-40066.
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AU - Lu, Yiling

AU - Yu, Qinghua

AU - Liu, Jue Hui

AU - Zhang, Jinyi

AU - Wang, Hongwei

AU - Koul, Dimpy

AU - McMurray, John S.

AU - Fang, Xianjun

AU - Yung, W. K Alfred

AU - Siminovitch, Kathy A.

AU - Mills, Gordon

PY - 2003/10/10

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N2 - Src family protein-tyrosine kinases, which play an important role in signal integration, have been implicated in tumorigenesis in multiple lineages, including breast cancer. We demonstrate, herein, that Src kinases regulate the phosphatidylinositol 3-kinase (PI3K) signaling cascade via altering the function of the PTEN tumor suppressor. Overexpression of activated Src protein-tyrosine kinases in PTEN-deficient breast cancer cells does not alter AKT phosphorylation, an indicator of signal transduction through the PI3K pathway. However, in the presence of functional PTEN, Src reverses the activity of PTEN, resulting in an increase in AKT phosphorylation. Activated Src reduces the ability of PTEN to dephosphorylate phosphatidylinositols in micelles and promotes AKT translocation to cellular plasma membranes but does not alter PTEN activity toward water-soluble phosphatidylinositols. Thus, Src may alter the capacity of the PTEN C2 domain to bind cellular membranes rather than directly interfering with PTEN enzymatic activity. Tyrosine phosphorylation of PTEN is increased in breast cancer cells treated with pervanadate, suggesting that PTEN contains sites for tyrosine phosphorylation. Src kinase inhibitors markedly decreased pervanadate-mediated tyrosine phosphorylation of PTEN. Further, expression of activated Src results in marked tyrosine phosphorylation of PTEN. SHP-1, a SH2 domain-containing protein-tyrosine phosphatase, selectively binds and dephosphorylates PTEN in Src transfected cells. Both Src inhibitors and SHP-1 overexpression reverse Src-induced loss of PTEN function. Coexpression of PTEN with activated Src reduces the stability of PTEN. Taken together, the data indicate that activated Src inhibits PTEN function leading to alterations in signaling through the PI3K/AKT pathway.

AB - Src family protein-tyrosine kinases, which play an important role in signal integration, have been implicated in tumorigenesis in multiple lineages, including breast cancer. We demonstrate, herein, that Src kinases regulate the phosphatidylinositol 3-kinase (PI3K) signaling cascade via altering the function of the PTEN tumor suppressor. Overexpression of activated Src protein-tyrosine kinases in PTEN-deficient breast cancer cells does not alter AKT phosphorylation, an indicator of signal transduction through the PI3K pathway. However, in the presence of functional PTEN, Src reverses the activity of PTEN, resulting in an increase in AKT phosphorylation. Activated Src reduces the ability of PTEN to dephosphorylate phosphatidylinositols in micelles and promotes AKT translocation to cellular plasma membranes but does not alter PTEN activity toward water-soluble phosphatidylinositols. Thus, Src may alter the capacity of the PTEN C2 domain to bind cellular membranes rather than directly interfering with PTEN enzymatic activity. Tyrosine phosphorylation of PTEN is increased in breast cancer cells treated with pervanadate, suggesting that PTEN contains sites for tyrosine phosphorylation. Src kinase inhibitors markedly decreased pervanadate-mediated tyrosine phosphorylation of PTEN. Further, expression of activated Src results in marked tyrosine phosphorylation of PTEN. SHP-1, a SH2 domain-containing protein-tyrosine phosphatase, selectively binds and dephosphorylates PTEN in Src transfected cells. Both Src inhibitors and SHP-1 overexpression reverse Src-induced loss of PTEN function. Coexpression of PTEN with activated Src reduces the stability of PTEN. Taken together, the data indicate that activated Src inhibits PTEN function leading to alterations in signaling through the PI3K/AKT pathway.

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