Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis

Shunji Nakano, Elke Küster-Schöck, Alan D. Grossman, Peter Zuber

Research output: Contribution to journalArticle

177 Citations (Scopus)

Abstract

The Spx protein of Bacillus subtilis represses activator-stimulated transcription by interacting with the C-terminal domain of RNA polymerase (RNAP) α subunit. Its concentration increases in cells lacking the ATP-dependent protease, ClpXP, resulting in severe effects on growth and developmental processes. Microarray analysis was undertaken to identify genes that are induced or repressed when Spx interacts with RNAP. The induced genes included those encoding products known to function in maintaining thiol homeostasis. Two genes, thioredoxin (trxA) and thioredoxin reductase (trxB), are transcriptionally induced under conditions of thiol-specific oxidative (disulfide) stress by a mechanism involving Spx-RNAP interaction. Disulfide stress also results in an increase in Spx-dependent transcriptional repression. The increase in Spx activity in cells encountering disulfide stress is due in part to a posttranscriptional mechanism of spx control resulting in an increase in Spx concentration. An spx null mutant and a strain bearing an allele of rpoA that prevents Spx-RNAP interaction show hypersensitivity to disulfide stress. From these results, it is proposed that Spx is an activator that mobilizes the operations necessary to reverse the effects of oxidative damage, but it also serves as a negative regulator that causes the postponement of developmental programs and energy-consuming growth-related functions while the cell copes with the period of stress.

Original languageEnglish (US)
Pages (from-to)13603-13608
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number23
DOIs
StatePublished - Nov 11 2003

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DNA-Directed RNA Polymerases
Bacillus subtilis
Sulfhydryl Compounds
Disulfides
Oxidative Stress
ATP-Dependent Proteases
Thioredoxin-Disulfide Reductase
Genes
Thioredoxins
Microarray Analysis
Growth
Hypersensitivity
Homeostasis
Alleles
Proteins

Keywords

  • ClpXP
  • Disulfide stress
  • Transcription activation

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Spx-dependent global transcriptional control is induced by thiol-specific oxidative stress in Bacillus subtilis. / Nakano, Shunji; Küster-Schöck, Elke; Grossman, Alan D.; Zuber, Peter.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 23, 11.11.2003, p. 13603-13608.

Research output: Contribution to journalArticle

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