Spontaneous in vitro production of anti-DNA and anti-RNA by systemic lupus erythematosus and normal peripheral blood mononuclear cells

Michael Liebling, C. Wong, J. Radosevich

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10 Scopus citations

Abstract

The spontaneous in vitro production of anti-DNA and anti-RNA by peripheral blood mononuclear cells (PBMC) from patients with systemic lupus erythematosus (SLE) and from normal subjects was evaluated employing sensitive solid-phase assays that are specific for these antibodies. PBMC from SLE patients produced more IgG anti-DNA and anti-RNA than did normal PBMC (P <0.01). In vitro production of IgG anti-DNA appeared to correlate with serum DNA bindings (r = 0.72, P <0.005). Similar amounts of IgM anti-DNA and anti-RNA were produced by both SLE and normal PBMC. However, IgM anti-DNA antibodies always appeared to be directed against determinants on denatured DNA. Only PBMC from SLE patients produced IgG antibodies to native DNA.

Original languageEnglish (US)
Pages (from-to)431-440
Number of pages10
JournalArthritis and Rheumatism
Volume28
Issue number4
Publication statusPublished - 1985
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Rheumatology

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