"Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II

James R. Florini, Karen A. Magri, Daina Z. Ewton, Payton L. James, Kent Grindstaff, Peter S. Rotwein

Research output: Contribution to journalArticle

312 Citations (Scopus)

Abstract

Differentiation of muscle cells to form postmitotic myotubes is usually viewed as being negatively controlled by medium components, sometimes designated "mitogens". However, we have found that a family of mitogenic agents, the insulin-like growth factors (IGFs), are potent stimulators of differentiation in myoblasts which act by inducing expression of the myogenin gene. We show here that this action of the IGFs occurs even when these growth factors are not added to the cell medium; upon transfer to low-serum "differentiation medium", myoblasts begin active expression of the IGF-II gene, at both the mRNA and protein levels. Furthermore, autocrine secretion of IGF-II is essential for the process of terminal differentiation of the cells. These conclusions are based upon four lines of evidence. (1) The rate of spontaneous differentiation in several sublines of myogenic cells correlates with their level of expression of IGF-II. (2) C2 and Sol 8 cells, which secrete high levels of IGF-II, are relatively insensitive to exogenous IGFs, in contrast to L6 lines, which exhibit lower levels of IGF-II gene expression. (3) An antisense oligodeoxyribonucleotide complementary to the first five codons of IGF-II inhibits myogenic differentiation in the absence but not in the presence of exogenous IGF-II. (4) Spontaneous differentiation in response to autocrine IGF-II involves the same mechanism that occurs in cells stimulated by the IGFs, i.e. elevation of expression of the myogenin gene.

Original languageEnglish (US)
Pages (from-to)15917-15923
Number of pages7
JournalJournal of Biological Chemistry
Volume266
Issue number24
StatePublished - 1991
Externally publishedYes

Fingerprint

Skeletal Myoblasts
Insulin-Like Growth Factor II
Somatomedins
Myogenin
Genes
Myoblasts
Gene Expression
Antisense Oligodeoxyribonucleotides
Cells
Serum-Free Culture Media
Skeletal Muscle Fibers
Polymethyl Methacrylate
Mitogens
Gene expression
Codon
Muscle Cells
Muscle
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

Florini, J. R., Magri, K. A., Ewton, D. Z., James, P. L., Grindstaff, K., & Rotwein, P. S. (1991). "Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. Journal of Biological Chemistry, 266(24), 15917-15923.

"Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. / Florini, James R.; Magri, Karen A.; Ewton, Daina Z.; James, Payton L.; Grindstaff, Kent; Rotwein, Peter S.

In: Journal of Biological Chemistry, Vol. 266, No. 24, 1991, p. 15917-15923.

Research output: Contribution to journalArticle

Florini, JR, Magri, KA, Ewton, DZ, James, PL, Grindstaff, K & Rotwein, PS 1991, '"Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II', Journal of Biological Chemistry, vol. 266, no. 24, pp. 15917-15923.
Florini, James R. ; Magri, Karen A. ; Ewton, Daina Z. ; James, Payton L. ; Grindstaff, Kent ; Rotwein, Peter S. / "Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II. In: Journal of Biological Chemistry. 1991 ; Vol. 266, No. 24. pp. 15917-15923.
@article{f7be5c48e6ce447ebc9d8c1bc07a1f3a,
title = "{"}Spontaneous{"} differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II",
abstract = "Differentiation of muscle cells to form postmitotic myotubes is usually viewed as being negatively controlled by medium components, sometimes designated {"}mitogens{"}. However, we have found that a family of mitogenic agents, the insulin-like growth factors (IGFs), are potent stimulators of differentiation in myoblasts which act by inducing expression of the myogenin gene. We show here that this action of the IGFs occurs even when these growth factors are not added to the cell medium; upon transfer to low-serum {"}differentiation medium{"}, myoblasts begin active expression of the IGF-II gene, at both the mRNA and protein levels. Furthermore, autocrine secretion of IGF-II is essential for the process of terminal differentiation of the cells. These conclusions are based upon four lines of evidence. (1) The rate of spontaneous differentiation in several sublines of myogenic cells correlates with their level of expression of IGF-II. (2) C2 and Sol 8 cells, which secrete high levels of IGF-II, are relatively insensitive to exogenous IGFs, in contrast to L6 lines, which exhibit lower levels of IGF-II gene expression. (3) An antisense oligodeoxyribonucleotide complementary to the first five codons of IGF-II inhibits myogenic differentiation in the absence but not in the presence of exogenous IGF-II. (4) Spontaneous differentiation in response to autocrine IGF-II involves the same mechanism that occurs in cells stimulated by the IGFs, i.e. elevation of expression of the myogenin gene.",
author = "Florini, {James R.} and Magri, {Karen A.} and Ewton, {Daina Z.} and James, {Payton L.} and Kent Grindstaff and Rotwein, {Peter S.}",
year = "1991",
language = "English (US)",
volume = "266",
pages = "15917--15923",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "24",

}

TY - JOUR

T1 - "Spontaneous" differentiation of skeletal myoblasts is dependent upon autocrine secretion of insulin-like growth factor-II

AU - Florini, James R.

AU - Magri, Karen A.

AU - Ewton, Daina Z.

AU - James, Payton L.

AU - Grindstaff, Kent

AU - Rotwein, Peter S.

PY - 1991

Y1 - 1991

N2 - Differentiation of muscle cells to form postmitotic myotubes is usually viewed as being negatively controlled by medium components, sometimes designated "mitogens". However, we have found that a family of mitogenic agents, the insulin-like growth factors (IGFs), are potent stimulators of differentiation in myoblasts which act by inducing expression of the myogenin gene. We show here that this action of the IGFs occurs even when these growth factors are not added to the cell medium; upon transfer to low-serum "differentiation medium", myoblasts begin active expression of the IGF-II gene, at both the mRNA and protein levels. Furthermore, autocrine secretion of IGF-II is essential for the process of terminal differentiation of the cells. These conclusions are based upon four lines of evidence. (1) The rate of spontaneous differentiation in several sublines of myogenic cells correlates with their level of expression of IGF-II. (2) C2 and Sol 8 cells, which secrete high levels of IGF-II, are relatively insensitive to exogenous IGFs, in contrast to L6 lines, which exhibit lower levels of IGF-II gene expression. (3) An antisense oligodeoxyribonucleotide complementary to the first five codons of IGF-II inhibits myogenic differentiation in the absence but not in the presence of exogenous IGF-II. (4) Spontaneous differentiation in response to autocrine IGF-II involves the same mechanism that occurs in cells stimulated by the IGFs, i.e. elevation of expression of the myogenin gene.

AB - Differentiation of muscle cells to form postmitotic myotubes is usually viewed as being negatively controlled by medium components, sometimes designated "mitogens". However, we have found that a family of mitogenic agents, the insulin-like growth factors (IGFs), are potent stimulators of differentiation in myoblasts which act by inducing expression of the myogenin gene. We show here that this action of the IGFs occurs even when these growth factors are not added to the cell medium; upon transfer to low-serum "differentiation medium", myoblasts begin active expression of the IGF-II gene, at both the mRNA and protein levels. Furthermore, autocrine secretion of IGF-II is essential for the process of terminal differentiation of the cells. These conclusions are based upon four lines of evidence. (1) The rate of spontaneous differentiation in several sublines of myogenic cells correlates with their level of expression of IGF-II. (2) C2 and Sol 8 cells, which secrete high levels of IGF-II, are relatively insensitive to exogenous IGFs, in contrast to L6 lines, which exhibit lower levels of IGF-II gene expression. (3) An antisense oligodeoxyribonucleotide complementary to the first five codons of IGF-II inhibits myogenic differentiation in the absence but not in the presence of exogenous IGF-II. (4) Spontaneous differentiation in response to autocrine IGF-II involves the same mechanism that occurs in cells stimulated by the IGFs, i.e. elevation of expression of the myogenin gene.

UR - http://www.scopus.com/inward/record.url?scp=0025993772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025993772&partnerID=8YFLogxK

M3 - Article

C2 - 1651927

AN - SCOPUS:0025993772

VL - 266

SP - 15917

EP - 15923

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 24

ER -