Specificity of T and B cell responses to bovine rhodopsin in Lewis rats

Edward J. Moticka, Grazyna Adamus

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Rhodopsin, an integral membrane protein of rod photoreceptor cells, induces an experimental autoimmune uveitis (EAU) when injected into Lewis rats. This disease is characterized by a mononuclear and polymorphonuclear cellular infiltrate of the retina resulting in destruction of the photoreceptor cells. In this study the B and T cell specificities of the response to bovine rhodopsin by Lewis rats were determined. Antibodies induced by injection of rhodopsin were directed almost exclusively to the IV-V loop (residues 174-202). Later in the response, antibody to the N-terminus was also detected. At the T cell level, most activity was directed to the C-terminus as measured by in vitro lymphocyte proliferation. Other minor T cell epitopes were found in the II-III (96-114) and IV-V (174-202) loops. Further dissection of the amino acid sequence responsible for the activity isolated to the C-terminus indicated that a 12-amino acid-long sequence (331-342) elicited the strongest proliferative response.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalCellular Immunology
Volume138
Issue number1
DOIs
StatePublished - Nov 1991

ASJC Scopus subject areas

  • Immunology

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