Specificity of T and B cell responses to bovine rhodopsin in Lewis rats

Edward J. Moticka, Grazyna Adamus

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Rhodopsin, an integral membrane protein of rod photoreceptor cells, induces an experimental autoimmune uveitis (EAU) when injected into Lewis rats. This disease is characterized by a mononuclear and polymorphonuclear cellular infiltrate of the retina resulting in destruction of the photoreceptor cells. In this study the B and T cell specificities of the response to bovine rhodopsin by Lewis rats were determined. Antibodies induced by injection of rhodopsin were directed almost exclusively to the IV-V loop (residues 174-202). Later in the response, antibody to the N-terminus was also detected. At the T cell level, most activity was directed to the C-terminus as measured by in vitro lymphocyte proliferation. Other minor T cell epitopes were found in the II-III (96-114) and IV-V (174-202) loops. Further dissection of the amino acid sequence responsible for the activity isolated to the C-terminus indicated that a 12-amino acid-long sequence (331-342) elicited the strongest proliferative response.

Original languageEnglish (US)
Pages (from-to)175-184
Number of pages10
JournalCellular Immunology
Volume138
Issue number1
DOIs
StatePublished - 1991
Externally publishedYes

Fingerprint

Rhodopsin
B-Lymphocytes
T-Lymphocytes
Amino Acid Sequence
T-Cell Antigen Receptor Specificity
Retinal Rod Photoreceptor Cells
Photoreceptor Cells
T-Lymphocyte Epitopes
Uveitis
Antibody Formation
Retina
Dissection
Membrane Proteins
Lymphocytes
Injections
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Specificity of T and B cell responses to bovine rhodopsin in Lewis rats. / Moticka, Edward J.; Adamus, Grazyna.

In: Cellular Immunology, Vol. 138, No. 1, 1991, p. 175-184.

Research output: Contribution to journalArticle

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