Specificity of anti-nucleoside antibodies in systemic lupus erythematosus

Richard H. Weisbart, Rebecca A. Garrett, Michael R. Liebling, Eugene V. Barnett, Harold E. Paulus, David H. Katz

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

The titer of IgG antinucleoside antibodies in the sera of 162 individuals was determined by an enzyme-linked immunosorbent assay. The nucleosides used in the assay were adenosine, cytidine, guanosine, and thymine-riboside conjugated to human serum albumin. The specificity of IgG antinucleoside antibodies was indicated by appropriate reduction in antibody binding after solid-phase adsorptions of antibody with specific immobilized nucleoside conjugates. Disease-associated increases in serum IgG antibodies to cytidine and guanosine but not to adenosine or thymine-riboside occurred in patients with systemic lupus erythematosus (SLE). The epitope density of nucleosides in the conjugates and differences in the sensitivity of each nucleoside assay were not responsible for disease-associated IgG antinucleoside antibody responses. These findings support a possible pathogenic role for cytidine and guanosine as antigens or crossreactive antigenic determinants in some patients with SLE.

Original languageEnglish (US)
Pages (from-to)403-411
Number of pages9
JournalClinical Immunology and Immunopathology
Volume27
Issue number3
DOIs
StatePublished - Jun 1983

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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