Specification of epibranchial placodes in zebrafish

Alex Nechiporuk, Tor Linbo, Kenneth D. Poss, David W. Raible

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

In all vertebrates, the neurogenic placodes are transient ectodermal thickenings that give rise to sensory neurons of the cranial ganglia. Epibranchial (EB) placodes generate neurons of the distal facial, glossopharyngeal and vagal ganglia, which convey sensation from the viscera, including pharyngeal endoderm structures, to the CNS. Recent studies have implicated signals from pharyngeal endoderm in the initiation of neurogenesis from EB placodes; however, the signals underlying the formation of placodes are unknown. Here, we show that zebrafish embryos mutant for fgf3 and fgf8 do not express early EB placode markers, including foxi1 and pax2a. Mosaic analysis demonstrates that placodal cells must directly receive Fgf signals during a specific crucial period of development. Transplantation experiments and mutant analysis reveal that cephalic mesoderm is the source of Fgf signals. Finally, both Fgf3 and Fgf8 are sufficient to induce foxi1-positive placodal precursors in wild-type as well as Fgf3- plus Fgf8-depleted embryos. We propose a model in which mesoderm-derived Fgf3 and Fgf8 signals establish both the EB placodes and the development of the pharyngeal endoderm, the subsequent interaction of which promotes neurogenesis. The coordinated interplay between craniofacial tissues would thus assure proper spatial and temporal interactions in the shaping of the vertebrate head.

Original languageEnglish (US)
Pages (from-to)611-623
Number of pages13
JournalDevelopment
Volume134
Issue number3
DOIs
StatePublished - Feb 2007
Externally publishedYes

Fingerprint

Endoderm
Zebrafish
Neurogenesis
Mesoderm
Ganglia
Vertebrates
Embryonic Structures
Head
Viscera
Sensory Receptor Cells
Transplantation
Neurons

Keywords

  • Cephalic mesoderm
  • Cranial ganglia
  • Epibranchial placodes
  • Fgf3
  • Fgf8
  • Foxi1
  • Pax2a
  • Zebrafish

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Specification of epibranchial placodes in zebrafish. / Nechiporuk, Alex; Linbo, Tor; Poss, Kenneth D.; Raible, David W.

In: Development, Vol. 134, No. 3, 02.2007, p. 611-623.

Research output: Contribution to journalArticle

Nechiporuk, A, Linbo, T, Poss, KD & Raible, DW 2007, 'Specification of epibranchial placodes in zebrafish', Development, vol. 134, no. 3, pp. 611-623. https://doi.org/10.1242/dev.02749
Nechiporuk, Alex ; Linbo, Tor ; Poss, Kenneth D. ; Raible, David W. / Specification of epibranchial placodes in zebrafish. In: Development. 2007 ; Vol. 134, No. 3. pp. 611-623.
@article{25ef24c67f244128a8d8d3c53e26518f,
title = "Specification of epibranchial placodes in zebrafish",
abstract = "In all vertebrates, the neurogenic placodes are transient ectodermal thickenings that give rise to sensory neurons of the cranial ganglia. Epibranchial (EB) placodes generate neurons of the distal facial, glossopharyngeal and vagal ganglia, which convey sensation from the viscera, including pharyngeal endoderm structures, to the CNS. Recent studies have implicated signals from pharyngeal endoderm in the initiation of neurogenesis from EB placodes; however, the signals underlying the formation of placodes are unknown. Here, we show that zebrafish embryos mutant for fgf3 and fgf8 do not express early EB placode markers, including foxi1 and pax2a. Mosaic analysis demonstrates that placodal cells must directly receive Fgf signals during a specific crucial period of development. Transplantation experiments and mutant analysis reveal that cephalic mesoderm is the source of Fgf signals. Finally, both Fgf3 and Fgf8 are sufficient to induce foxi1-positive placodal precursors in wild-type as well as Fgf3- plus Fgf8-depleted embryos. We propose a model in which mesoderm-derived Fgf3 and Fgf8 signals establish both the EB placodes and the development of the pharyngeal endoderm, the subsequent interaction of which promotes neurogenesis. The coordinated interplay between craniofacial tissues would thus assure proper spatial and temporal interactions in the shaping of the vertebrate head.",
keywords = "Cephalic mesoderm, Cranial ganglia, Epibranchial placodes, Fgf3, Fgf8, Foxi1, Pax2a, Zebrafish",
author = "Alex Nechiporuk and Tor Linbo and Poss, {Kenneth D.} and Raible, {David W.}",
year = "2007",
month = "2",
doi = "10.1242/dev.02749",
language = "English (US)",
volume = "134",
pages = "611--623",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "3",

}

TY - JOUR

T1 - Specification of epibranchial placodes in zebrafish

AU - Nechiporuk, Alex

AU - Linbo, Tor

AU - Poss, Kenneth D.

AU - Raible, David W.

PY - 2007/2

Y1 - 2007/2

N2 - In all vertebrates, the neurogenic placodes are transient ectodermal thickenings that give rise to sensory neurons of the cranial ganglia. Epibranchial (EB) placodes generate neurons of the distal facial, glossopharyngeal and vagal ganglia, which convey sensation from the viscera, including pharyngeal endoderm structures, to the CNS. Recent studies have implicated signals from pharyngeal endoderm in the initiation of neurogenesis from EB placodes; however, the signals underlying the formation of placodes are unknown. Here, we show that zebrafish embryos mutant for fgf3 and fgf8 do not express early EB placode markers, including foxi1 and pax2a. Mosaic analysis demonstrates that placodal cells must directly receive Fgf signals during a specific crucial period of development. Transplantation experiments and mutant analysis reveal that cephalic mesoderm is the source of Fgf signals. Finally, both Fgf3 and Fgf8 are sufficient to induce foxi1-positive placodal precursors in wild-type as well as Fgf3- plus Fgf8-depleted embryos. We propose a model in which mesoderm-derived Fgf3 and Fgf8 signals establish both the EB placodes and the development of the pharyngeal endoderm, the subsequent interaction of which promotes neurogenesis. The coordinated interplay between craniofacial tissues would thus assure proper spatial and temporal interactions in the shaping of the vertebrate head.

AB - In all vertebrates, the neurogenic placodes are transient ectodermal thickenings that give rise to sensory neurons of the cranial ganglia. Epibranchial (EB) placodes generate neurons of the distal facial, glossopharyngeal and vagal ganglia, which convey sensation from the viscera, including pharyngeal endoderm structures, to the CNS. Recent studies have implicated signals from pharyngeal endoderm in the initiation of neurogenesis from EB placodes; however, the signals underlying the formation of placodes are unknown. Here, we show that zebrafish embryos mutant for fgf3 and fgf8 do not express early EB placode markers, including foxi1 and pax2a. Mosaic analysis demonstrates that placodal cells must directly receive Fgf signals during a specific crucial period of development. Transplantation experiments and mutant analysis reveal that cephalic mesoderm is the source of Fgf signals. Finally, both Fgf3 and Fgf8 are sufficient to induce foxi1-positive placodal precursors in wild-type as well as Fgf3- plus Fgf8-depleted embryos. We propose a model in which mesoderm-derived Fgf3 and Fgf8 signals establish both the EB placodes and the development of the pharyngeal endoderm, the subsequent interaction of which promotes neurogenesis. The coordinated interplay between craniofacial tissues would thus assure proper spatial and temporal interactions in the shaping of the vertebrate head.

KW - Cephalic mesoderm

KW - Cranial ganglia

KW - Epibranchial placodes

KW - Fgf3

KW - Fgf8

KW - Foxi1

KW - Pax2a

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=33847340216&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847340216&partnerID=8YFLogxK

U2 - 10.1242/dev.02749

DO - 10.1242/dev.02749

M3 - Article

C2 - 17215310

AN - SCOPUS:33847340216

VL - 134

SP - 611

EP - 623

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 3

ER -