Specific suppression of anti-DNA production in vitro

Michael R. Liebling, Curtice Wong, Jean Radosevich, James S. Louie

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

To investigate the regulation of anti-DNA antibody production, we generated anti-DNA-specific suppressor cells by exposing normal human T cells and a small percentage of adherent cells to high concentrations of DNA. These cells suppressed the production of anti-DNA by both autologous peripheral blood mononuclear cells (PBMC) and allogeneic PBMC derived from systemic lupus erythematosus (SLE) patients. Anti-DNA production was suppressed significantly more than anti-RNA, antitetanus, or total immunoglobulin production. Specific suppression was enhanced by increasing the numbers of DNA-primed CD8+ cells and was obliterated by irradiation of the DNA-primed cells. In contrast to T cells from normal individuals, T cells obtained from two intensively studied SLE patients were unable to generate specific suppressor cells for anti-DNA production in both autologous and allogeneic test systems. Despite this defect, these patients were still capable of generating specific suppressor cells for antibody production directed against an exogenous antigen, tetanus toxoid.

Original languageEnglish (US)
Pages (from-to)362-371
Number of pages10
JournalJournal of Clinical Immunology
Volume8
Issue number5
DOIs
StatePublished - Sep 1 1988

Keywords

  • Anti-DNA
  • T cells
  • specific suppression
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Liebling, M. R., Wong, C., Radosevich, J., & Louie, J. S. (1988). Specific suppression of anti-DNA production in vitro. Journal of Clinical Immunology, 8(5), 362-371. https://doi.org/10.1007/BF00917152