Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult β-globin gene

Beverly Emerson, G. Felsenfeld

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number1 I
DOIs
StatePublished - Jan 1 1984
Externally publishedYes

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Globins
Chickens
Hypersensitivity
Genes
5' Flanking Region
Cell Extracts
Histones
Chromatin
Digestion
Plasmids
Erythrocytes
DNA

ASJC Scopus subject areas

  • General

Cite this

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AU - Felsenfeld, G.

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N2 - Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

AB - Chromatin in the 5' flanking region of the transcriptionally active chicken adult β (β(A) globin genes is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the β(A)-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

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