Specific and nonspecific effects of ethanol vapor on plasma corticosterone in mice

L. Donald Keith, John Jr Crabbe

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The intent of this study was to determine whether chronic ethanol (EtOH) vapor inhalation, with or without adjunct pyrazole (PYR) administration, was stressful in mice, as defined by increases in plasma corticosterone (CORT) concentration. Mice were randomly assigned to groups differentiated both on the basis of EtOH vapor exposure and the presence or absence of PYR administration. Blood samples for blood EtOH concentration (BEC) and plasma CORT concentration were obtained from mice after 72-96 hours of treatment. Mice were sacrificed after 96 hours of treatment and body and adrenal weight determined. BEC was significantly higher in PYR-treated animals and animals treated with the higher EtOH vapor concentration. Plasma CORT was elevated in proportion to BEC; however, other nonspecific stresses, in particular that of PYR administration, also elevated plasma CORT. Nonspecific stresses associated with this protocol may reduce the generality of these observations. Nevertheless, the high correlation between BEC and plasma CORT concentration in the PYR groups indicates that, with suitable control groups, the PYR-EtOH vapor inhalation approach is viable for studies concerned with EtOH effects on hypothalamic-anterior pituitary-adrenocortical function.

Original languageEnglish (US)
Pages (from-to)529-533
Number of pages5
JournalAlcohol
Volume9
Issue number6
DOIs
StatePublished - 1992

Fingerprint

Corticosterone
Ethanol
Vapors
Blood
Plasmas
animal
Group
Inhalation
Animals
pyrazole
Body Weight
Control Groups

Keywords

  • Corticosterone
  • Ethanol
  • Mice
  • Stress

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Neuroscience(all)
  • Behavioral Neuroscience
  • Toxicology
  • Health(social science)

Cite this

Specific and nonspecific effects of ethanol vapor on plasma corticosterone in mice. / Keith, L. Donald; Crabbe, John Jr.

In: Alcohol, Vol. 9, No. 6, 1992, p. 529-533.

Research output: Contribution to journalArticle

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