TY - JOUR
T1 - Speciation and biosynthetic variation in four dictyoceratid sponges and their cyanobacterial symbiont, Oscillatoria spongeliae
AU - Ridley, Christian P.
AU - Bergquist, Patricia R.
AU - Harper, Mary Kay
AU - Faulkner, D. John
AU - Hooper, John N.A.
AU - Haygood, Margo G.
N1 - Funding Information:
We thank the Coral Reef Research Foundation (CRRF) for their invaluable assistance in collecting these sponges, the Republic of Palau for the research permits, Catherine Sincich and Koty Sharp for taking the underwater photographs, and Christine Salomon for assistance with molecular tools and field work. Mark Hildebrand assisted with the cesium chloride/Hoechst dye gradient, and Grace Lim provided advice on experiments and phylogenetic analysis. We especially thank Bill Gerwick and Patty Flatt (Oregon State University) for generously providing the halogenase primers prior to publishing them and Bill Fenical for his support of this project. The transmission electron microscopy was completed with Pat Reid at the VA San Diego Hospital (La Jolla), and Matt Woolery assisted with the mass spectrometry. The DNA sequencing was performed by the DNA Sequencing Shared Resource, UCSD Cancer Center, which is funded in part by NCI Cancer Center Support Grant 2 P30 CA23100-18. This research was supported by a grant from the National Science Foundation (CHE 98-16169).
PY - 2005/3
Y1 - 2005/3
N2 - Four species of marine sponges (Phylum Porifera, Order Dictyoceratida), which contain the filamentous cyanobacterial symbiont Oscillatoria spongeliae, were collected from four locations in Palau. The halogenated natural products associated with the symbiont were characterized from each sample, revealing that each species contained either chlorinated peptides, brominated diphenyl ethers, or no halogenated compounds. Analysis of the host sponges and the symbionts indicated that each species of sponge contained a distinct strain of morphologically similar cyanobacteria. Although cospeciation may be present in this group, we have identified that at least one host switching event has occurred in this symbiosis. Only the strain of O. spongeliae in the sponge containing the chlorinated compounds possessed genes involved in the biosynthesis of chlorinated leucine precursors, indicating that the chemical variation observed in these animals has a genetic foundation.
AB - Four species of marine sponges (Phylum Porifera, Order Dictyoceratida), which contain the filamentous cyanobacterial symbiont Oscillatoria spongeliae, were collected from four locations in Palau. The halogenated natural products associated with the symbiont were characterized from each sample, revealing that each species contained either chlorinated peptides, brominated diphenyl ethers, or no halogenated compounds. Analysis of the host sponges and the symbionts indicated that each species of sponge contained a distinct strain of morphologically similar cyanobacteria. Although cospeciation may be present in this group, we have identified that at least one host switching event has occurred in this symbiosis. Only the strain of O. spongeliae in the sponge containing the chlorinated compounds possessed genes involved in the biosynthesis of chlorinated leucine precursors, indicating that the chemical variation observed in these animals has a genetic foundation.
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U2 - 10.1016/j.chembiol.2005.02.003
DO - 10.1016/j.chembiol.2005.02.003
M3 - Article
C2 - 15797223
AN - SCOPUS:15744365948
SN - 2451-9448
VL - 12
SP - 397
EP - 406
JO - Cell Chemical Biology
JF - Cell Chemical Biology
IS - 3
ER -