Spatial variability overwhelms seasonal patterns in bacterioplankton communities across a river to ocean gradient

Caroline S. Fortunato, Lydie Herfort, Peter Zuber, Antonio M. Baptista, Byron C. Crump

Research output: Contribution to journalArticle

152 Scopus citations

Abstract

Few studies of microbial biogeography address variability across both multiple habitats and multiple seasons. Here we examine the spatial and temporal variability of bacterioplankton community composition of the Columbia River coastal margin using 16S amplicon pyrosequencing of 300 water samples collected in 2007 and 2008. Communities separated into seven groups (ANOSIM, P<0.001): river, estuary, plume, epipelagic, mesopelagic, shelf bottom (depth<350 m) and slope bottom (depth>850 m). The ordination of these samples was correlated with salinity (ρ=-0.83) and depth (ρ=-0.62). Temporal patterns were obscured by spatial variability among the coastal environments, and could only be detected within individual groups. Thus, structuring environmental factors (for example, salinity, depth) dominate over seasonal changes in determining community composition. Seasonal variability was detected across an annual cycle in the river, estuary and plume where communities separated into two groups, early year (April-July) and late year (August-Nov), demonstrating annual reassembly of communities over time. Determining both the spatial and temporal variability of bacterioplankton communities provides a framework for modeling these communities across environmental gradients from river to deep ocean.

Original languageEnglish (US)
Pages (from-to)554-563
Number of pages10
JournalISME Journal
Volume6
Issue number3
DOIs
StatePublished - Mar 2012

Keywords

  • 16S amplicon pyrosequencing
  • Columbia River
  • coastal ocean
  • gradient
  • spatial variability
  • temporal variability

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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