TY - JOUR
T1 - Spatial variability overwhelms seasonal patterns in bacterioplankton communities across a river to ocean gradient
AU - Fortunato, Caroline S.
AU - Herfort, Lydie
AU - Zuber, Peter
AU - Baptista, Antonio M.
AU - Crump, Byron C.
N1 - Funding Information:
This study was carried out within the context of the Science and Technology Center for Coastal Margin Observation & Prediction supported by the National Science Foundation (grant number OCE-0424602 to Antonio Baptista). We would like to thank the captains and crews of the R/V Wecoma and R/V Barnes, chief scientists Murray Levine, Tawnya Peterson and Joe Needoba, as well as Suzanne DeLorenzo, Peter Kahn and Daniel Murphy for their help in sample collection. We also thank Joe Jones and John Busch for 454-pyrosequen-cing support, and Patrick Schloss and Jack Gilbert for their help in pyrosequence analysis.
PY - 2012/3
Y1 - 2012/3
N2 - Few studies of microbial biogeography address variability across both multiple habitats and multiple seasons. Here we examine the spatial and temporal variability of bacterioplankton community composition of the Columbia River coastal margin using 16S amplicon pyrosequencing of 300 water samples collected in 2007 and 2008. Communities separated into seven groups (ANOSIM, P<0.001): river, estuary, plume, epipelagic, mesopelagic, shelf bottom (depth<350 m) and slope bottom (depth>850 m). The ordination of these samples was correlated with salinity (ρ=-0.83) and depth (ρ=-0.62). Temporal patterns were obscured by spatial variability among the coastal environments, and could only be detected within individual groups. Thus, structuring environmental factors (for example, salinity, depth) dominate over seasonal changes in determining community composition. Seasonal variability was detected across an annual cycle in the river, estuary and plume where communities separated into two groups, early year (April-July) and late year (August-Nov), demonstrating annual reassembly of communities over time. Determining both the spatial and temporal variability of bacterioplankton communities provides a framework for modeling these communities across environmental gradients from river to deep ocean.
AB - Few studies of microbial biogeography address variability across both multiple habitats and multiple seasons. Here we examine the spatial and temporal variability of bacterioplankton community composition of the Columbia River coastal margin using 16S amplicon pyrosequencing of 300 water samples collected in 2007 and 2008. Communities separated into seven groups (ANOSIM, P<0.001): river, estuary, plume, epipelagic, mesopelagic, shelf bottom (depth<350 m) and slope bottom (depth>850 m). The ordination of these samples was correlated with salinity (ρ=-0.83) and depth (ρ=-0.62). Temporal patterns were obscured by spatial variability among the coastal environments, and could only be detected within individual groups. Thus, structuring environmental factors (for example, salinity, depth) dominate over seasonal changes in determining community composition. Seasonal variability was detected across an annual cycle in the river, estuary and plume where communities separated into two groups, early year (April-July) and late year (August-Nov), demonstrating annual reassembly of communities over time. Determining both the spatial and temporal variability of bacterioplankton communities provides a framework for modeling these communities across environmental gradients from river to deep ocean.
KW - 16S amplicon pyrosequencing
KW - Columbia River
KW - coastal ocean
KW - gradient
KW - spatial variability
KW - temporal variability
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U2 - 10.1038/ismej.2011.135
DO - 10.1038/ismej.2011.135
M3 - Article
C2 - 22011718
AN - SCOPUS:84857117285
SN - 1751-7362
VL - 6
SP - 554
EP - 563
JO - ISME Journal
JF - ISME Journal
IS - 3
ER -