Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans

Stuart Ibsen, Ada Tong, Carolyn Schutt, Sadik Esener, Sreekanth H. Chalasani

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

A major challenge in neuroscience is to reliably activate individual neurons, particularly those in deeper brain regions. Current optogenetic approaches require invasive surgical procedures to deliver light of specific wavelengths to target cells to activate or silence them. Here, we demonstrate the use of low-pressure ultrasound as a non-invasive trigger to activate specific ultrasonically sensitized neurons in the nematode, Caenorhabditis elegans. We first show that wild-type animals are insensitive to low-pressure ultrasound and require gas-filled microbubbles to transduce the ultrasound wave. We find that neuron-specific misexpression of TRP-4, the pore-forming subunit of a mechanotransduction channel, sensitizes neurons to ultrasound stimulus, resulting in behavioural outputs. Furthermore, we use this approach to manipulate the function of sensory neurons and interneurons and identify a role for PVD sensory neurons in modifying locomotory behaviours. We suggest that this method can be broadly applied to manipulate cellular functions in vivo.

Original languageEnglish (US)
Article number8264
JournalNature Communications
Volume6
DOIs
StatePublished - Sep 15 2015
Externally publishedYes

Fingerprint

Caenorhabditis elegans
neurons
Neurons
Sensory Receptor Cells
Ultrasonics
Optogenetics
Pressure
Microbubbles
Wild Animals
Interneurons
Neurosciences
low pressure
neurology
Gases
Physical vapor deposition
Light
stimuli
brain
animals
Brain

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Sonogenetics is a non-invasive approach to activating neurons in Caenorhabditis elegans. / Ibsen, Stuart; Tong, Ada; Schutt, Carolyn; Esener, Sadik; Chalasani, Sreekanth H.

In: Nature Communications, Vol. 6, 8264, 15.09.2015.

Research output: Contribution to journalArticle

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