Song presentation induces gene expression in the songbird forebrain

Claudio Mello, David S. Vicario, David F. Clayton

Research output: Contribution to journalArticle

461 Citations (Scopus)

Abstract

We investigated the participation of genomic regulatory events in the response of the songbird brain to a natural auditory stimulus of known physiological and behavioral relevance, birdsong. Using in situ hybridization, we detected a rapid increase in forebrain mRNA levels of an immediate-early gene encoding a transcriptional regulator (ZENK; also known as zif-268, egr-1, NGFI-A, or Krox-24) following presentation of tape-recorded songs to canaries (Serinus canaria) and zebra finches (Taeniopygia guttata). ZENK induction is most marked in a forebrain region believed to participate in auditory processing and is greatest when birds hear the song of their own species. A significantly lower level of induction occurs when birds hear the song of a different species and no induction is seen after exposure to tone bursts. Cellular analysis indicates that the level of induction reflects the proportion of neurons recruited to express the gene. These results suggest a role for genomic responses in neural processes linked to song pattern recognition, discrimination, or the formation of auditory associations.

Original languageEnglish (US)
Pages (from-to)6818-6822
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number15
StatePublished - 1992
Externally publishedYes

Fingerprint

Songbirds
Music
Prosencephalon
Canaries
Gene Expression
Birds
Finches
Immediate-Early Genes
Equidae
In Situ Hybridization
Neurons
Messenger RNA
Brain
Genes

Keywords

  • Auditory
  • Avian neostriatum
  • Immediate-early gene
  • Natural stimulus
  • Species recognition

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Song presentation induces gene expression in the songbird forebrain. / Mello, Claudio; Vicario, David S.; Clayton, David F.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 15, 1992, p. 6818-6822.

Research output: Contribution to journalArticle

@article{36524c3338644e61857e43cc2045cbf6,
title = "Song presentation induces gene expression in the songbird forebrain",
abstract = "We investigated the participation of genomic regulatory events in the response of the songbird brain to a natural auditory stimulus of known physiological and behavioral relevance, birdsong. Using in situ hybridization, we detected a rapid increase in forebrain mRNA levels of an immediate-early gene encoding a transcriptional regulator (ZENK; also known as zif-268, egr-1, NGFI-A, or Krox-24) following presentation of tape-recorded songs to canaries (Serinus canaria) and zebra finches (Taeniopygia guttata). ZENK induction is most marked in a forebrain region believed to participate in auditory processing and is greatest when birds hear the song of their own species. A significantly lower level of induction occurs when birds hear the song of a different species and no induction is seen after exposure to tone bursts. Cellular analysis indicates that the level of induction reflects the proportion of neurons recruited to express the gene. These results suggest a role for genomic responses in neural processes linked to song pattern recognition, discrimination, or the formation of auditory associations.",
keywords = "Auditory, Avian neostriatum, Immediate-early gene, Natural stimulus, Species recognition",
author = "Claudio Mello and Vicario, {David S.} and Clayton, {David F.}",
year = "1992",
language = "English (US)",
volume = "89",
pages = "6818--6822",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "15",

}

TY - JOUR

T1 - Song presentation induces gene expression in the songbird forebrain

AU - Mello, Claudio

AU - Vicario, David S.

AU - Clayton, David F.

PY - 1992

Y1 - 1992

N2 - We investigated the participation of genomic regulatory events in the response of the songbird brain to a natural auditory stimulus of known physiological and behavioral relevance, birdsong. Using in situ hybridization, we detected a rapid increase in forebrain mRNA levels of an immediate-early gene encoding a transcriptional regulator (ZENK; also known as zif-268, egr-1, NGFI-A, or Krox-24) following presentation of tape-recorded songs to canaries (Serinus canaria) and zebra finches (Taeniopygia guttata). ZENK induction is most marked in a forebrain region believed to participate in auditory processing and is greatest when birds hear the song of their own species. A significantly lower level of induction occurs when birds hear the song of a different species and no induction is seen after exposure to tone bursts. Cellular analysis indicates that the level of induction reflects the proportion of neurons recruited to express the gene. These results suggest a role for genomic responses in neural processes linked to song pattern recognition, discrimination, or the formation of auditory associations.

AB - We investigated the participation of genomic regulatory events in the response of the songbird brain to a natural auditory stimulus of known physiological and behavioral relevance, birdsong. Using in situ hybridization, we detected a rapid increase in forebrain mRNA levels of an immediate-early gene encoding a transcriptional regulator (ZENK; also known as zif-268, egr-1, NGFI-A, or Krox-24) following presentation of tape-recorded songs to canaries (Serinus canaria) and zebra finches (Taeniopygia guttata). ZENK induction is most marked in a forebrain region believed to participate in auditory processing and is greatest when birds hear the song of their own species. A significantly lower level of induction occurs when birds hear the song of a different species and no induction is seen after exposure to tone bursts. Cellular analysis indicates that the level of induction reflects the proportion of neurons recruited to express the gene. These results suggest a role for genomic responses in neural processes linked to song pattern recognition, discrimination, or the formation of auditory associations.

KW - Auditory

KW - Avian neostriatum

KW - Immediate-early gene

KW - Natural stimulus

KW - Species recognition

UR - http://www.scopus.com/inward/record.url?scp=0026636761&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026636761&partnerID=8YFLogxK

M3 - Article

C2 - 1495970

AN - SCOPUS:0026636761

VL - 89

SP - 6818

EP - 6822

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 15

ER -