Somatostatin is targeted to the regulated secretory pathway of gonadotrophs in transgenic mice expressing a metallothionein-somatostatin gene

M. J. Low, Philip Stork, R. E. Hammer, R. L. Brinster, M. J. Warhol, Gail Mandel, Richard Goodman

Research output: Contribution to journalArticle

6 Scopus citations


The pituitaries of transgenic mice that express a metallothionein-somatostatin fusion gene contain high concentrations of somatostatin-14 exclusively in the gonadotrophic cells. The purpose of this study was to determine whether somatostatin expressed from the foreign fusion gene enters the normal secretory pathway within these cells. Immuno-gold labeling of serial thin sections localized somatostatin to the secretory granules of gonadotropin-producing cells. The gonadotroph-specific hypophysiotropic factor, luteinizing hormone-releasing hormone caused a dose-dependent secretion of somatostatin when applied to primary pituitary cultures from these mice. Growth hormone-releasing hormone, thyrotropin-releasing hormone, corticotropin releasing factor, and dopamine did not affect somatostatin secretion. These experiments demonstrate that a neurosecretory peptide encoded by a foreign gene can enter the regulated secretory pathway of pituitary cells from transgenic mice.

Original languageEnglish (US)
Pages (from-to)16260-16263
Number of pages4
JournalJournal of Biological Chemistry
Issue number34
Publication statusPublished - 1986
Externally publishedYes


ASJC Scopus subject areas

  • Biochemistry

Cite this