Soluble granzyme B and cytotoxic T lymphocyte activity in the pathogenesis of systemic lupus erythematosus

Dilip Shah, Ravi Kiran, Ajay Wanchu, Archana Bhatnagar

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Activated cytotoxic T lymphocyte (CTL) mediated target cell death has been implicated in the development of systemic autoimmune disease like SLE. However, the role of soluble granzyme B and its relationship with CTL activity and disease activity is still unknown. In this study, we evaluated role of soluble granzyme B and cytotoxic T lymphocyte activity in SLE patients. The soluble granzyme B was measured in the serum by an enzyme-linked immunosorbent assay while cytotoxic T lymphocyte activity was measured by flow cytometry. The disease activity was determined by using SLE Disease Activity Index (SLEDAI) score. Cytotoxic T lymphocyte activity was increased and strongly associated with disease activity. The soluble granzyme B levels were higher in SLE patients and associated with various clinical features like reduced complement components; C3 & C4 and skin lesion. The soluble granzyme B levels were also sturdily related with severity of the disease. The findings of this study suggest that excessive secretion of soluble granzyme B and enhanced activity of cytotoxic T lymphocyte may play a vital role in the pathogenesis of SLE and organ damage. Also, evaluation of soluble granzyme B may be helpful in monitoring the clinical features associated with activated CTL in SLE.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalCellular Immunology
Volume269
Issue number1
DOIs
StatePublished - 2011

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Granzymes
Cytotoxic T-Lymphocytes
Systemic Lupus Erythematosus
Autoimmune Diseases
Flow Cytometry
Cell Death
Enzyme-Linked Immunosorbent Assay
Skin
Serum

Keywords

  • Cytotoxic T lymphocyte
  • SLEDAI score
  • Soluble granzyme B
  • Systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology

Cite this

Soluble granzyme B and cytotoxic T lymphocyte activity in the pathogenesis of systemic lupus erythematosus. / Shah, Dilip; Kiran, Ravi; Wanchu, Ajay; Bhatnagar, Archana.

In: Cellular Immunology, Vol. 269, No. 1, 2011, p. 16-21.

Research output: Contribution to journalArticle

Shah, Dilip ; Kiran, Ravi ; Wanchu, Ajay ; Bhatnagar, Archana. / Soluble granzyme B and cytotoxic T lymphocyte activity in the pathogenesis of systemic lupus erythematosus. In: Cellular Immunology. 2011 ; Vol. 269, No. 1. pp. 16-21.
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