Sodium transport-related proteins in the mammalian distal nephron - Distribution, ontogeny and functional aspects

Sebastian Bachmann, Magdalena Bostanjoglo, Roland Schmitt, David Ellison

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The mammalian distal nephron plays a pivotal role in adjusting urinary sodium excretion. Successive portions of the renal tubule are formed to adapt to this function, and an axial heterogeneity of the distal segments has been defined. The specific transport properties of these epithelia are accomplished by the expression of proteins (cotransporters, exchangers, channels) governing the movement of ions on either cell side. Molecular cloning of these proteins has had a marked impact on the study of their localization and function in the healthy and diseased kidney. Electroneutral cation-chloride cotransporters [Na(K)CC] have been localized to the thick ascending limb and the distal convoluted tubule using specific probes. Proteins implicated in the function of aldosterone target cells, such as the epithelial Na+ channel (ENaC), the mineralocorticoid receptor (MR) and 11β-hydroxysteroid dehydrogenase type 2 (11HSD2), an enzyme that confers mineralocorticoid specificity, have been found in the terminal portion of the nephron and the collecting duct. A mineralocorticoid-sensitive component of thiazide-sensitive NaCl transport has been identified in the distal convoluted tubule. Analysis of the ontogeny of these proteins in the maturing kidney has provided a detailed picture of epithelial differentiation and morphological specialization of the renal tubule. The study of mutations of the proteins related with NaCl transport has led to the identification of the molecular causes of inherited human diseases associated with hypo- or hypertension, and the respective sites of an impaired ion transport could be mapped to the renal tubule.

Original languageEnglish (US)
Pages (from-to)447-468
Number of pages22
JournalAnatomy and Embryology
Volume200
Issue number5
DOIs
StatePublished - 1999
Externally publishedYes

Fingerprint

Nephrons
Carrier Proteins
Sodium
Kidney
Mineralocorticoids
Proteins
11-beta-Hydroxysteroid Dehydrogenases
Epithelial Sodium Channels
Thiazides
Mineralocorticoid Receptors
Ion Transport
Kidney Diseases
Molecular Cloning
Aldosterone
Hypotension
Cations
Chlorides
Extremities
Epithelium
Ions

Keywords

  • Homeostasis
  • Kidney
  • Renal tubule
  • Sodium excretion

ASJC Scopus subject areas

  • Anatomy
  • Developmental Biology
  • Cell Biology
  • Embryology

Cite this

Sodium transport-related proteins in the mammalian distal nephron - Distribution, ontogeny and functional aspects. / Bachmann, Sebastian; Bostanjoglo, Magdalena; Schmitt, Roland; Ellison, David.

In: Anatomy and Embryology, Vol. 200, No. 5, 1999, p. 447-468.

Research output: Contribution to journalArticle

Bachmann, Sebastian ; Bostanjoglo, Magdalena ; Schmitt, Roland ; Ellison, David. / Sodium transport-related proteins in the mammalian distal nephron - Distribution, ontogeny and functional aspects. In: Anatomy and Embryology. 1999 ; Vol. 200, No. 5. pp. 447-468.
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