Sod2 knockdown in the musculature has whole-organism consequences in Drosophila

Ian Martin, Melanie A. Jones, Devin Rhodenizer, Jie Zheng, John M. Warrick, Laurent Seroude, Mike Grotewiel

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Oxidative damage to cell macromolecules by reactive oxygen species is associated with numerous diseases and aging. In Drosophila, RNAi-mediated silencing of the mitochondrial antioxidant manganese superoxide dismutase (SOD2) throughout the body dramatically reduces life span, accelerates senescence of locomotor function, and enhances sensitivity to applied oxidative stress. Here, we show that Sod2 knockdown in the musculature alone is sufficient to cause the shortened life span and accelerated locomotor declines observed with knockdown of Sod2 throughout the body, indicating that Sod2 deficiency in muscle is central to these phenotypes. Knockdown of Sod2 in the muscle also increased caspase activity (a marker for apoptosis) and caused a mitochondrial pathology characterized by swollen mitochondria, decreased mitochondrial content, and reduced ATP levels. These findings indicate that Sod2 plays a crucial role in the musculature in Drosophila and that the consequences of SOD2 loss in this tissue extend to the viability of the organism as a whole.

Original languageEnglish (US)
Pages (from-to)803-813
Number of pages11
JournalFree Radical Biology and Medicine
Volume47
Issue number6
DOIs
StatePublished - Sep 15 2009
Externally publishedYes

Fingerprint

Drosophila
Muscle
Muscles
Mitochondria
Oxidative stress
Pathology
Caspases
RNA Interference
Macromolecules
Superoxide Dismutase
Reactive Oxygen Species
Oxidative Stress
Antioxidants
Adenosine Triphosphate
Aging of materials
Tissue
Apoptosis
Phenotype

Keywords

  • Free radicals
  • Mitochondria
  • Muscle
  • SOD2

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Martin, I., Jones, M. A., Rhodenizer, D., Zheng, J., Warrick, J. M., Seroude, L., & Grotewiel, M. (2009). Sod2 knockdown in the musculature has whole-organism consequences in Drosophila. Free Radical Biology and Medicine, 47(6), 803-813. https://doi.org/10.1016/j.freeradbiomed.2009.06.021

Sod2 knockdown in the musculature has whole-organism consequences in Drosophila. / Martin, Ian; Jones, Melanie A.; Rhodenizer, Devin; Zheng, Jie; Warrick, John M.; Seroude, Laurent; Grotewiel, Mike.

In: Free Radical Biology and Medicine, Vol. 47, No. 6, 15.09.2009, p. 803-813.

Research output: Contribution to journalArticle

Martin, I, Jones, MA, Rhodenizer, D, Zheng, J, Warrick, JM, Seroude, L & Grotewiel, M 2009, 'Sod2 knockdown in the musculature has whole-organism consequences in Drosophila', Free Radical Biology and Medicine, vol. 47, no. 6, pp. 803-813. https://doi.org/10.1016/j.freeradbiomed.2009.06.021
Martin, Ian ; Jones, Melanie A. ; Rhodenizer, Devin ; Zheng, Jie ; Warrick, John M. ; Seroude, Laurent ; Grotewiel, Mike. / Sod2 knockdown in the musculature has whole-organism consequences in Drosophila. In: Free Radical Biology and Medicine. 2009 ; Vol. 47, No. 6. pp. 803-813.
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