Social stress exacerbates stroke outcome by suppressing Bcl-2 expression

A. Courtney DeVries, Hung Dong Joh, Ora Bernard, Kimihiko Hattori, Patricia D. Hurn, Richard J. Traystman, Nabil Alkayed

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

The relationship between stressful life events and the onset of disease is well documented. However, the role of psychological stress as a risk factor for life-threatening cerebrovascular insults such as stroke remains unspecified, but could explain individual variation in stroke outcome. To discover the mechanisms through which psychological stress may alter stroke outcome, we modeled the effects of chronic social intimidation and stress on ischemiainduced bcl-2 expression and early neuronal cell loss resulting from cerebral artery occlusion in mice (C57BL/6). The bcl-2 protooncogene promotes cell survival and protects against apoptosis and cellular necrosis in numerous neurodegenerative disorders, including stroke. In our study, male mice were chronically exposed to aggressive social stimuli before induction of a controlled, mild ischemic insult. Stressed mice expressed ≈70% less bcl-2 mRNA than unstressed mice after ischemia. In addition, social stress greatly exacerbated infarct in wild-type mice but not in transgenic mice that constitutively express increased neuronal bcl-2. Despite similar postischemic concentrations of corticosterone, the major stress hormone in mice, high corticosterone concentrations were significantly correlated with larger infarcts in wild-type mice but not bcl-2 transgenic mice. Thus, enhanced bcl-2 expression offsets the potentially deleterious consequences of high postischemic plasma corticosterone concentrations. Taken together, these data demonstrate that stressful prestroke social milieu strongly compromises an endogenous molecular mechanism of neuroprotection in injured brain and offer a new behavioral target for stroke therapy.

Original languageEnglish (US)
Pages (from-to)11824-11828
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number20
DOIs
StatePublished - Sep 25 2001
Externally publishedYes

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Stroke
Corticosterone
Psychological Stress
Transgenic Mice
Cerebral Arteries
Inbred C57BL Mouse
Neurodegenerative Diseases
Cell Survival
Necrosis
Ischemia
Hormones
Apoptosis
Messenger RNA
Brain
Therapeutics

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Social stress exacerbates stroke outcome by suppressing Bcl-2 expression. / DeVries, A. Courtney; Joh, Hung Dong; Bernard, Ora; Hattori, Kimihiko; Hurn, Patricia D.; Traystman, Richard J.; Alkayed, Nabil.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, No. 20, 25.09.2001, p. 11824-11828.

Research output: Contribution to journalArticle

DeVries, A. Courtney ; Joh, Hung Dong ; Bernard, Ora ; Hattori, Kimihiko ; Hurn, Patricia D. ; Traystman, Richard J. ; Alkayed, Nabil. / Social stress exacerbates stroke outcome by suppressing Bcl-2 expression. In: Proceedings of the National Academy of Sciences of the United States of America. 2001 ; Vol. 98, No. 20. pp. 11824-11828.
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