Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures

D. A. Galloway, Jay Nelson, J. K. McDougall

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the BglII N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a M(r) 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.

Original languageEnglish (US)
Pages (from-to)4736-4740
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number15 I
StatePublished - 1984
Externally publishedYes

Fingerprint

Insertional Mutagenesis
Herpesviridae
DNA Transposable Elements
Human Herpesvirus 2
Nucleic Acid Repetitive Sequences
DNA
Cytomegalovirus
Base Pairing
Rodentia
Hand
Peptides
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures. / Galloway, D. A.; Nelson, Jay; McDougall, J. K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 15 I, 1984, p. 4736-4740.

Research output: Contribution to journalArticle

@article{f624a08b874640a2a33f53ddc4d5ced5,
title = "Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures",
abstract = "A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the BglII N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a M(r) 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.",
author = "Galloway, {D. A.} and Jay Nelson and McDougall, {J. K.}",
year = "1984",
language = "English (US)",
volume = "81",
pages = "4736--4740",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "15 I",

}

TY - JOUR

T1 - Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures

AU - Galloway, D. A.

AU - Nelson, Jay

AU - McDougall, J. K.

PY - 1984

Y1 - 1984

N2 - A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the BglII N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a M(r) 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.

AB - A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the BglII N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a M(r) 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0021235397&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021235397&partnerID=8YFLogxK

M3 - Article

C2 - 6087345

AN - SCOPUS:0021235397

VL - 81

SP - 4736

EP - 4740

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 15 I

ER -