Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures

D. A. Galloway, J. A. Nelson, J. K. McDougall

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the BglII N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a M(r) 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.

Original languageEnglish (US)
Pages (from-to)4736-4740
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number15 I
DOIs
StatePublished - Jan 1 1984

ASJC Scopus subject areas

  • General

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