TY - JOUR
T1 - Small-conductance calcium-activated K+ channels are expressed in pancreatic islets and regulate glucose responses
AU - Tamarina, Natalia A.
AU - Wang, Yong
AU - Mariotto, Loris
AU - Kuznetsov, Andrey
AU - Bond, Chris
AU - Adelman, John
AU - Philipson, Louis H.
PY - 2003/8/1
Y1 - 2003/8/1
N2 - Glucose-stimulated insulin secretion is associated with transients of intracellular Ca2+ concentration [Ca2+]i in the pancreatic β-cell. We identified the expression and function of specific small-conductance Ca2+-activated K+ (SK) channel genes in insulin-secreting cells. The presence of mRNA for SK1, -2, -3, and -4 (intermediate-conductance Ca2+-activated K+ 1 [IK1]) channels was demonstrated by RT-PCR in rodent islets and insulinoma cells. SK2 and -3 proteins in mouse islets were detected by immunoblot and immunocytochemistry. In the tTA-SK3 tet-off mouse, a normal amount of SK3 protein was present in islets, but it became undetectable after exposure to doxycycline (DOX), which inhibits the transcription of the tTA-SK3 gene. The SK/IK channel-blockers apamin, dequalinium, and charybdotoxin caused increases in average [Ca2+]i levels and in frequency of [Ca2+]i oscillations in wild-type mouse islets. In SK3-tTA tet-off mice, the addition of apamin with glucose and tetraethylammonium (TEA) caused a similar elevation in [Ca2+]i, which was greatly diminished after DOX suppression of SK3 expression. We conclude that SK1, -2, -3, and IK1 (SK4) are expressed in islet cells and insulin-secreting cells and are able to influence glucose-induced calcium responses, thereby regulating insulin secretion.
AB - Glucose-stimulated insulin secretion is associated with transients of intracellular Ca2+ concentration [Ca2+]i in the pancreatic β-cell. We identified the expression and function of specific small-conductance Ca2+-activated K+ (SK) channel genes in insulin-secreting cells. The presence of mRNA for SK1, -2, -3, and -4 (intermediate-conductance Ca2+-activated K+ 1 [IK1]) channels was demonstrated by RT-PCR in rodent islets and insulinoma cells. SK2 and -3 proteins in mouse islets were detected by immunoblot and immunocytochemistry. In the tTA-SK3 tet-off mouse, a normal amount of SK3 protein was present in islets, but it became undetectable after exposure to doxycycline (DOX), which inhibits the transcription of the tTA-SK3 gene. The SK/IK channel-blockers apamin, dequalinium, and charybdotoxin caused increases in average [Ca2+]i levels and in frequency of [Ca2+]i oscillations in wild-type mouse islets. In SK3-tTA tet-off mice, the addition of apamin with glucose and tetraethylammonium (TEA) caused a similar elevation in [Ca2+]i, which was greatly diminished after DOX suppression of SK3 expression. We conclude that SK1, -2, -3, and IK1 (SK4) are expressed in islet cells and insulin-secreting cells and are able to influence glucose-induced calcium responses, thereby regulating insulin secretion.
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U2 - 10.2337/diabetes.52.8.2000
DO - 10.2337/diabetes.52.8.2000
M3 - Article
C2 - 12882916
AN - SCOPUS:0042266303
SN - 0012-1797
VL - 52
SP - 2000
EP - 2006
JO - Diabetes
JF - Diabetes
IS - 8
ER -