Small-conductance Ca2+-activated K+ channel type 2 (SK2) modulates hippocampal learning, memory, and synaptic plasticity

Rebecca S. Hammond, Chris T. Bond, Timothy Strassmaier, Thu Jennifer Ngo-Anh, John Adelman, James Maylie, Robert W. Stackman

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Apamin-sensitive, small-conductance, Ca2+-activated K + channels (SK channels) modulate neuronal excitability in CA1 neurons. Blocking all SK channel subtypes with apamin facilitates the induction of hippocampal synaptic plasticity and enhances hippocampal learning. In CA1 dendrites, SK channels are activated by Ca2+ through NMDA receptors and restrict glutamate-mediated EPSPs. Studies of SK channel knock-out mice reveal that of the three apamin-sensitive SK channel subunits (SK1-SK3), only SK2 subunits are necessary for the apamin-sensitive currents in CA1 hippocampal neurons. To determine the specific influence of SK2 channels on hippocampal synaptic plasticity, learning, and memory, we used gene targeting through homologous recombination in embryonic stem cells to generate transgenic mice that overexpress SK2 subunits by 10-fold (SK2+/T). In these mice, the apamin-sensitive current in CA1 neurons was increased by approximately fourfold, relative to wild-type (WT) littermates. In addition, the amplitude of synaptically evoked EPSPs recorded from SK2+/T CA1 neurons increased twice as much in response to SK channel blockade relative to EPSPs recorded from WT CA1 neurons. Consistent with this, SK2 overexpression reduced long-term potentiation after high-frequency stimulation compared with WT littermates and severely impaired learning in both hippocampus- and amygdala-dependent tasks. We conclude that SK2 channels regulate hippocampal synaptic plasticity and play a critical role in modulating mechanisms of learning and memory.

Original languageEnglish (US)
Pages (from-to)1844-1853
Number of pages10
JournalJournal of Neuroscience
Volume26
Issue number6
DOIs
StatePublished - Feb 8 2006

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Calcium-Activated Potassium Channels
Neuronal Plasticity
Apamin
Learning
Excitatory Postsynaptic Potentials
Neurons
Gene Targeting
Long-Term Potentiation
Homologous Recombination
Embryonic Stem Cells
Dendrites
Amygdala
N-Methyl-D-Aspartate Receptors
Knockout Mice
Transgenic Mice
Glutamic Acid
Hippocampus

Keywords

  • Fear conditioning
  • Hippocampus
  • Long-term potentiation
  • Potassium channel
  • Spatial learning
  • Spatial memory

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Small-conductance Ca2+-activated K+ channel type 2 (SK2) modulates hippocampal learning, memory, and synaptic plasticity. / Hammond, Rebecca S.; Bond, Chris T.; Strassmaier, Timothy; Ngo-Anh, Thu Jennifer; Adelman, John; Maylie, James; Stackman, Robert W.

In: Journal of Neuroscience, Vol. 26, No. 6, 08.02.2006, p. 1844-1853.

Research output: Contribution to journalArticle

Hammond, Rebecca S. ; Bond, Chris T. ; Strassmaier, Timothy ; Ngo-Anh, Thu Jennifer ; Adelman, John ; Maylie, James ; Stackman, Robert W. / Small-conductance Ca2+-activated K+ channel type 2 (SK2) modulates hippocampal learning, memory, and synaptic plasticity. In: Journal of Neuroscience. 2006 ; Vol. 26, No. 6. pp. 1844-1853.
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