Slow changes of tyrosine hydroxylase gene expression in dopaminergic brain neurons after neurotoxin lesioning: a model for neuron aging

G. M. Pasinetti, H. H. Osterburg, A. B. Kelly, Steven Kohama, D. G. Morgan, J. F. Reinhard, R. H. Stellwagen, C. E. Finch

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Slow neuron regression develops during the adult phase of life in select brain systems of mammals. We describe a model in adult rats that resolves several phases in a slow atrophic process that differentially influences levels of mRNA and protein for tyrosine hydroxylase (TH). Responses of striatal dopaminergic markers to 6-hydroxydopamine (6-OHDA) lesions in rats indicated that the striatal terminals maintained TH protein, despite >3-fold loss of TH mRNA in the substantia nigra pars compacta (SNC) cell bodies whose axons project to the striatum. The loss of TH mRNA/cell was progressive up to 9 months, whereas SNC cell body shrinkage stabilized by 3 months post-lesioning. Consideration of possible mechanisms in protein turnover motivated a search for PEST motifs in the TH of rats and other vertebrates that could be a point of regulation by altering the rate of TH protein turnover.

Original languageEnglish (US)
Pages (from-to)63-73
Number of pages11
JournalMolecular Brain Research
Volume13
Issue number1-2
DOIs
StatePublished - 1992
Externally publishedYes

Fingerprint

Dopaminergic Neurons
Neurotoxins
Tyrosine 3-Monooxygenase
Gene Expression
Neurons
Brain
Corpus Striatum
Oxidopamine
Messenger RNA
Proteins
Axons
Vertebrates
Mammals

Keywords

  • 6-Hydroxydopamine
  • Aging
  • Basal ganglia
  • Dopamine
  • mRNA regulation
  • PEST sequence
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Pasinetti, G. M., Osterburg, H. H., Kelly, A. B., Kohama, S., Morgan, D. G., Reinhard, J. F., ... Finch, C. E. (1992). Slow changes of tyrosine hydroxylase gene expression in dopaminergic brain neurons after neurotoxin lesioning: a model for neuron aging. Molecular Brain Research, 13(1-2), 63-73. https://doi.org/10.1016/0169-328X(92)90045-D

Slow changes of tyrosine hydroxylase gene expression in dopaminergic brain neurons after neurotoxin lesioning : a model for neuron aging. / Pasinetti, G. M.; Osterburg, H. H.; Kelly, A. B.; Kohama, Steven; Morgan, D. G.; Reinhard, J. F.; Stellwagen, R. H.; Finch, C. E.

In: Molecular Brain Research, Vol. 13, No. 1-2, 1992, p. 63-73.

Research output: Contribution to journalArticle

Pasinetti, GM, Osterburg, HH, Kelly, AB, Kohama, S, Morgan, DG, Reinhard, JF, Stellwagen, RH & Finch, CE 1992, 'Slow changes of tyrosine hydroxylase gene expression in dopaminergic brain neurons after neurotoxin lesioning: a model for neuron aging', Molecular Brain Research, vol. 13, no. 1-2, pp. 63-73. https://doi.org/10.1016/0169-328X(92)90045-D
Pasinetti, G. M. ; Osterburg, H. H. ; Kelly, A. B. ; Kohama, Steven ; Morgan, D. G. ; Reinhard, J. F. ; Stellwagen, R. H. ; Finch, C. E. / Slow changes of tyrosine hydroxylase gene expression in dopaminergic brain neurons after neurotoxin lesioning : a model for neuron aging. In: Molecular Brain Research. 1992 ; Vol. 13, No. 1-2. pp. 63-73.
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