Slit-scan flow cytometry: analysis of Chinese hamster M3-1 chromosomes

J. W. Gray, J. Lucas, D. Pinkel

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3 Scopus citations

Abstract

We have applied slit-scan flow cytometry to the measurement of the distribution of fluorescence along isolated Chinese hamster M3-1 chromosomes stained with ethidium bromide. One hundred chromosome profiles were measured for each of the chromosome numbers 1, 2, 4 + t (X; 5) and 5. DNA based centromeric indices calculated for these chromosomes are 0.54 ± 0.02, 0.52 ± 0.02, 0.57 ± 0.03 and 0.57 ± 0.03, respectively. These values are in good agreement with DNA based centomeric indices calculated for the same chromosomes using chromosome profiles extracted from scanning cytophotometric images of Chinese hamster M3-1 metaphase spreads stained with gallocyanin chrome alum. The pulse shapes from the Chinese hamster chromosomes in flow were found to be approximately constant in height and to fall within a pulse width range encompassing the largest and smallest chromosomes. Pulse height and width discrimination substantially reduced the contribution of non chromosomal debris to the total fluorescence distribution (flow karyotype).

Original languageEnglish (US)
Pages (from-to)249-255
Number of pages7
JournalActa Pathologica et Microbiologica Scandinavica - Section A Pathology
Volume89
Issue numberSuppl.274
StatePublished - Jan 1 1981

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Microbiology (medical)

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