Slit-scan flow cytometry: analysis of Chinese hamster M3-1 chromosomes

Joe Gray, J. Lucas, D. Pinkel

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

We have applied slit-scan flow cytometry to the measurement of the distribution of fluorescence along isolated Chinese hamster M3-1 chromosomes stained with ethidium bromide. One hundred chromosome profiles were measured for each of the chromosome numbers 1, 2, 4 + t (X; 5) and 5. DNA based centromeric indices calculated for these chromosomes are 0.54 ± 0.02, 0.52 ± 0.02, 0.57 ± 0.03 and 0.57 ± 0.03, respectively. These values are in good agreement with DNA based centomeric indices calculated for the same chromosomes using chromosome profiles extracted from scanning cytophotometric images of Chinese hamster M3-1 metaphase spreads stained with gallocyanin chrome alum. The pulse shapes from the Chinese hamster chromosomes in flow were found to be approximately constant in height and to fall within a pulse width range encompassing the largest and smallest chromosomes. Pulse height and width discrimination substantially reduced the contribution of non chromosomal debris to the total fluorescence distribution (flow karyotype).

Original languageEnglish (US)
Title of host publicationActa Pathologica et Microbiologica Scandinavica - Section A Pathology
Pages249-255
Number of pages7
Volume89
EditionSuppl.274
StatePublished - 1981
Externally publishedYes

Fingerprint

Chromosomes, Human, Pair 1
Cricetulus
Flow Cytometry
Chromosomes
Fluorescence
Ethidium
DNA
Metaphase
Karyotype

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Gray, J., Lucas, J., & Pinkel, D. (1981). Slit-scan flow cytometry: analysis of Chinese hamster M3-1 chromosomes. In Acta Pathologica et Microbiologica Scandinavica - Section A Pathology (Suppl.274 ed., Vol. 89, pp. 249-255)

Slit-scan flow cytometry : analysis of Chinese hamster M3-1 chromosomes. / Gray, Joe; Lucas, J.; Pinkel, D.

Acta Pathologica et Microbiologica Scandinavica - Section A Pathology. Vol. 89 Suppl.274. ed. 1981. p. 249-255.

Research output: Chapter in Book/Report/Conference proceedingChapter

Gray, J, Lucas, J & Pinkel, D 1981, Slit-scan flow cytometry: analysis of Chinese hamster M3-1 chromosomes. in Acta Pathologica et Microbiologica Scandinavica - Section A Pathology. Suppl.274 edn, vol. 89, pp. 249-255.
Gray J, Lucas J, Pinkel D. Slit-scan flow cytometry: analysis of Chinese hamster M3-1 chromosomes. In Acta Pathologica et Microbiologica Scandinavica - Section A Pathology. Suppl.274 ed. Vol. 89. 1981. p. 249-255
Gray, Joe ; Lucas, J. ; Pinkel, D. / Slit-scan flow cytometry : analysis of Chinese hamster M3-1 chromosomes. Acta Pathologica et Microbiologica Scandinavica - Section A Pathology. Vol. 89 Suppl.274. ed. 1981. pp. 249-255
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