Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation

Rodrigo N. Romcy-Pereira, Loubna Erraji-Benchekroun, Peggy Smyrniotopoulos, Sonoko Ogawa, Claudio Mello, Etienne Sibille, Constantine Pavlides

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The activity-dependent transcription factor zif268 is re-activated in sleep following hippocampal long-term potentiation (LTP). However, the activation of secondary genes, possibly involved in modifying local synaptic strengths and ultimately stabilizing memory traces during sleep, has not yet been studied. Here, we investigated changes in hippocampal and cortical gene expression at a time point subsequent to the previously reported initial zif268 re-activation during sleep. Rats underwent unilateral hippocampal LTP and were assigned to SLEEP or AWAKE groups. Eighty minutes after a long rapid-eye-movement sleep (REMS) episode (or an equivalent amount of time for awake group) animals had their hippocampi dissected and processed for gene microarray hybridization. Prefrontal and parietal cortices were also collected for qRT-PCR analysis. The microarray analysis identified 28 up-regulated genes in the hippocampus: 11 genes were enhanced in the LTPed hemisphere of sleep animals; 13 genes were enhanced after sleep, regardless of hemisphere; and 4 genes were enhanced in LTPed hemisphere, regardless of behavioral state. qRT-PCR analysis confirmed the up-regulation of aif-1 and sc-65 during sleep. Moreover, we observed a down-regulation of the purinergic receptor, P2Y4R in the LTP hemisphere of awake animals and a trend for the protein kinase, CaMKI to be up-regulated in the LTP hemisphere of sleep animals. In the prefrontal cortex, we showed a significant LTP-dependent down-regulation of gluR1 and spinophilin specifically during sleep. Zif268 was down-regulated in sleep regardless of the hemisphere. No changes in gene expression were observed in the parietal cortex. Our findings indicate that a set of synaptic plasticity-related genes have their expression modulated during sleep following LTP, which can reflect biochemical events associated with reshaping of synaptic connections in sleep following learning.

Original languageEnglish (US)
Pages (from-to)44-52
Number of pages9
JournalPhysiology and Behavior
Volume98
Issue number1-2
DOIs
StatePublished - Aug 4 2009

Fingerprint

Long-Term Potentiation
Prefrontal Cortex
Hippocampus
Sleep
Gene Expression
Parietal Lobe
Genes
Down-Regulation
Purinergic Receptors
Polymerase Chain Reaction
Pseudogenes
Neuronal Plasticity
REM Sleep
Microarray Analysis
Protein Kinases
Transcriptional Activation
Transcription Factors
Up-Regulation
Learning

Keywords

  • Gene expression
  • Hippocampus
  • Long-term potentiation (LTP)
  • Microarray analysis
  • Prefrontal cortex (PFC)
  • Rapid-eye movement (REM) sleep

ASJC Scopus subject areas

  • Behavioral Neuroscience
  • Experimental and Cognitive Psychology

Cite this

Romcy-Pereira, R. N., Erraji-Benchekroun, L., Smyrniotopoulos, P., Ogawa, S., Mello, C., Sibille, E., & Pavlides, C. (2009). Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation. Physiology and Behavior, 98(1-2), 44-52. https://doi.org/10.1016/j.physbeh.2009.04.010

Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation. / Romcy-Pereira, Rodrigo N.; Erraji-Benchekroun, Loubna; Smyrniotopoulos, Peggy; Ogawa, Sonoko; Mello, Claudio; Sibille, Etienne; Pavlides, Constantine.

In: Physiology and Behavior, Vol. 98, No. 1-2, 04.08.2009, p. 44-52.

Research output: Contribution to journalArticle

Romcy-Pereira, RN, Erraji-Benchekroun, L, Smyrniotopoulos, P, Ogawa, S, Mello, C, Sibille, E & Pavlides, C 2009, 'Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation', Physiology and Behavior, vol. 98, no. 1-2, pp. 44-52. https://doi.org/10.1016/j.physbeh.2009.04.010
Romcy-Pereira, Rodrigo N. ; Erraji-Benchekroun, Loubna ; Smyrniotopoulos, Peggy ; Ogawa, Sonoko ; Mello, Claudio ; Sibille, Etienne ; Pavlides, Constantine. / Sleep-dependent gene expression in the hippocampus and prefrontal cortex following long-term potentiation. In: Physiology and Behavior. 2009 ; Vol. 98, No. 1-2. pp. 44-52.
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