SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells

Vidya Murthy, Stéphane F. Maison, Julián Taranda, Nadeem Haque, Chris T. Bond, A. Belén Elgoyhen, John Adelman, M. Charles Liberman, Douglas E. Vetter

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Cochlear hair cells use SK2 currents to shape responses to cholinergic efferent feedback from the brain. Using SK2-/- mice, we demonstrate that, in addition to their previously defined role in modulating hair cell membrane potentials, SK2 channels are necessary for long-term survival of olivocochlear fibers and synapses. Loss of the SK2 gene also results in loss of electrically driven olivocochlear effects in vivo, and down regulation of ryanodine receptors involved in calcium-induced calcium release, the main inducer of nAChR evoked SK2 activity. Generation of double-null mice lacking both the α10 nAChR gene, loss of which results in hypertrophied olivocochlear terminals, and the SK2 gene, recapitulates the SK2-/- synaptic phenotype and gene expression, and also leads to down regulation of α9 nAChR gene expression. The data suggest a hierarchy of activity necessary to maintain early olivocochlear synapses at their targets, with SK2 serving an epistatic, upstream, role to the nAChRs.

Original languageEnglish (US)
Pages (from-to)39-49
Number of pages11
JournalMolecular and Cellular Neuroscience
Volume40
Issue number1
DOIs
StatePublished - Jan 5 2009

Fingerprint

Outer Auditory Hair Cells
Synapses
Auditory Hair Cells
Down-Regulation
Genes
Calcium
Gene Expression
Ryanodine Receptor Calcium Release Channel
Membrane Potentials
Cholinergic Agents
Cell Membrane
Phenotype
Brain

Keywords

  • Cochlea
  • Nicotinic receptors
  • Small conductance potassium channels
  • Synaptic degeneration
  • Synaptogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Cell Biology

Cite this

SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells. / Murthy, Vidya; Maison, Stéphane F.; Taranda, Julián; Haque, Nadeem; Bond, Chris T.; Elgoyhen, A. Belén; Adelman, John; Liberman, M. Charles; Vetter, Douglas E.

In: Molecular and Cellular Neuroscience, Vol. 40, No. 1, 05.01.2009, p. 39-49.

Research output: Contribution to journalArticle

Murthy, V, Maison, SF, Taranda, J, Haque, N, Bond, CT, Elgoyhen, AB, Adelman, J, Liberman, MC & Vetter, DE 2009, 'SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells', Molecular and Cellular Neuroscience, vol. 40, no. 1, pp. 39-49. https://doi.org/10.1016/j.mcn.2008.08.011
Murthy, Vidya ; Maison, Stéphane F. ; Taranda, Julián ; Haque, Nadeem ; Bond, Chris T. ; Elgoyhen, A. Belén ; Adelman, John ; Liberman, M. Charles ; Vetter, Douglas E. / SK2 channels are required for function and long-term survival of efferent synapses on mammalian outer hair cells. In: Molecular and Cellular Neuroscience. 2009 ; Vol. 40, No. 1. pp. 39-49.
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