SK2 channel plasticity contributes to LTP at Schaffer collateral-CA1 synapses

Mike T. Lin, Rafael Luján, Masahiko Watanabe, John Adelman, James Maylie

Research output: Contribution to journalArticle

140 Citations (Scopus)

Abstract

Long-term potentiation (LTP) of synaptic strength at Schaffer collateral synapses has largely been attributed to changes in the number and biophysical properties of AMPA receptors (AMPARs). Small-conductance Ca2+- activated K+ channels (SK2 channels) are functionally coupled with NMDA receptors (NMDARs) in CA1 spines such that their activity modulates the shape of excitatory postsynaptic potentials (EPSPs) and increases the threshold for induction of LTP. Here we show that LTP induction in mouse hippocampus abolishes SK2 channel activity in the potentiated synapses. This effect is due to SK2 channel internalization from the postsynaptic density (PSD) into the spine. Blocking PKA or cell dialysis with a peptide representing the C-terminal domain of SK2 that contains three known PKA phosphorylation sites blocks the internalization of SK2 channels after LTP induction. Thus the increase in AMPARs and the decrease in SK2 channels combine to produce the increased EPSP underlying LTP.

Original languageEnglish (US)
Pages (from-to)170-177
Number of pages8
JournalNature Neuroscience
Volume11
Issue number2
DOIs
StatePublished - Feb 2008

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Long-Term Potentiation
Synapses
Hippocampus
AMPA Receptors
Excitatory Postsynaptic Potentials
Spine
Post-Synaptic Density
Calcium-Activated Potassium Channels
N-Methyl-D-Aspartate Receptors
Dialysis
Phosphorylation

ASJC Scopus subject areas

  • Neuroscience(all)

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SK2 channel plasticity contributes to LTP at Schaffer collateral-CA1 synapses. / Lin, Mike T.; Luján, Rafael; Watanabe, Masahiko; Adelman, John; Maylie, James.

In: Nature Neuroscience, Vol. 11, No. 2, 02.2008, p. 170-177.

Research output: Contribution to journalArticle

Lin, Mike T. ; Luján, Rafael ; Watanabe, Masahiko ; Adelman, John ; Maylie, James. / SK2 channel plasticity contributes to LTP at Schaffer collateral-CA1 synapses. In: Nature Neuroscience. 2008 ; Vol. 11, No. 2. pp. 170-177.
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