SK2 and SK3 expression differentially affect firing frequency and precision in dopamine neurons

J. Deignan, R. Luján, C. Bond, A. Riegel, M. Watanabe, John Williams, James Maylie, John Adelman

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The firing properties of dopamine (DA) neurons in the substantia nigra (SN) pars compacta are strongly influenced by the activity of apamin-sensitive small conductance Ca2+-activated K+ (SK) channels. Of the three SK channel genes expressed in central neurons, only SK3 expression has been identified in DA neurons. The present findings show that SK2 was also expressed in DA neurons. Immuno-electron microscopy (iEM) showed that SK2 was primarily expressed in the distal dendrites, while SK3 was heavily expressed in the soma and, to a lesser extent, throughout the dendritic arbor. Electrophysiological recordings of the effects of the SK channel blocker apamin on DA neurons from wild type and SK-/- mice show that SK2-containing channels contributed to the precision of action potential (AP) timing, while SK3-containing channels influenced AP frequency. The expression of SK2 in DA neurons may endow distinct signaling and subcellular localization to SK2-containing channels.

Original languageEnglish (US)
Pages (from-to)67-76
Number of pages10
JournalNeuroscience
Volume217
DOIs
StatePublished - Aug 16 2012

Fingerprint

Dopaminergic Neurons
Apamin
Action Potentials
Calcium-Activated Potassium Channels
Immunoelectron Microscopy
Carisoprodol
Dendrites
Neurons
Genes

Keywords

  • Dopamine
  • Pacemaker
  • SK channels
  • Spontaneous activity
  • Substantia nigra

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

SK2 and SK3 expression differentially affect firing frequency and precision in dopamine neurons. / Deignan, J.; Luján, R.; Bond, C.; Riegel, A.; Watanabe, M.; Williams, John; Maylie, James; Adelman, John.

In: Neuroscience, Vol. 217, 16.08.2012, p. 67-76.

Research output: Contribution to journalArticle

Deignan, J. ; Luján, R. ; Bond, C. ; Riegel, A. ; Watanabe, M. ; Williams, John ; Maylie, James ; Adelman, John. / SK2 and SK3 expression differentially affect firing frequency and precision in dopamine neurons. In: Neuroscience. 2012 ; Vol. 217. pp. 67-76.
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AU - Williams, John

AU - Maylie, James

AU - Adelman, John

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