Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity

Christine Ulmke; Jens Kreth; Joseph W. Lengeler; Wolfram Welte; Kurt Schmid

doi:10.1128/jb.181.6.1920-1923.1999

Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity

Christine Ulmke, Jens Kreth, Joseph W. Lengeler, Wolfram Welte, Kurt Schmid

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.

Original language	English (US)
Pages (from-to)	1920-1923
Number of pages	4
Journal	Journal of bacteriology
Volume	181
Issue number	6
DOIs	https://doi.org/10.1128/jb.181.6.1920-1923.1999
State	Published - Mar 1999
Externally published	Yes

ASJC Scopus subject areas

Microbiology
Molecular Biology

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10.1128/jb.181.6.1920-1923.1999

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abstract = "The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.",

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T1 - Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity

AU - Ulmke, Christine

AU - Kreth, Jens

AU - Lengeler, Joseph W.

AU - Welte, Wolfram

AU - Schmid, Kurt

PY - 1999/3

Y1 - 1999/3

N2 - The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.

AB - The difference in substrate selectivity of the maltodextrin (LamB) and sucrose (ScrY) porins is attributed mainly to differences in loop L3, which is supposed to constrict the lumen of the pores. We show that even a single mutation (D201Y) in loop L3 leads to a narrowing of the substrate range of ScrY to that resembling LamB. In addition, we removed the putative N-terminal coiled-coil structure of ScrY and studied the effect of this deletion on sucrose transport.

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JO - Journal of bacteriology

JF - Journal of bacteriology

IS - 6

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Site-directed mutagenesis of loop L3 of sucrose porin ScrY leads to changes in substrate selectivity

Abstract

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