Single-stranded DNA aptamers against TNF and their potential applications

Shao Tao, Pingfang Song, Xiaowei Zhang, Lingshu Zhang, Cong Qiu Chu

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Aptamers are short, single-stranded RNA or DNA sequences, which can bind to protein ligands with high affinity and specificity. Applications of aptamers are broad, ranging from drugs and drug delivery vehicles to biosensors. Tumor necrosis factor (TNF) is an inflammatory cytokine that plays a critical role in the pathogenesis of several autoimmune inflammatory diseases. Blocking TNF activity by monoclonal antibodies or TNF receptor fusion protein has been tremendously successful in treating these diseases. However, manufacturing these biological TNF inhibitors is expensive and a significant proportion of patients do not respond to TNF blockade. Here we describe selection of single-stranded DNA aptamers for TNF blockage, and their bioactivity in blocking TNF-mediated cytotoxicity in vitro. These TNF-binding aptamers have the potential to serve as alternatives to biological TNF inhibitors and to be used as in vivo probes for TNF detection.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-196
Number of pages16
DOIs
StatePublished - Jan 1 2020

Publication series

NameMethods in Molecular Biology
Volume2108
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Aptamers
  • In vivo imaging
  • SELEX
  • ssDNA
  • Therapeutics
  • Tumor necrosis factor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Single-stranded DNA aptamers against TNF and their potential applications'. Together they form a unique fingerprint.

  • Cite this

    Tao, S., Song, P., Zhang, X., Zhang, L., & Chu, C. Q. (2020). Single-stranded DNA aptamers against TNF and their potential applications. In Methods in Molecular Biology (pp. 181-196). (Methods in Molecular Biology; Vol. 2108). Humana Press Inc.. https://doi.org/10.1007/978-1-0716-0247-8_16