Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells

N. W. Richards, D. C. Dawson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The patch-clamp technique for recording single-channel currents across cell membranes was applied to single turtle colon epithelial cells isolated with hyaluronidase. With electrodes fabricated from Corning #7052 glass, high-resistance seals were consistently formed to these cells. In on-cell patches with low K (2.5 mM) in the pipette and high K (114.5 mM) in the bath, outward K currents were recorded that had a slope conductance of 17 pS and a reversal potential greater than -70 mV. Currents through this K channel were blocked by lidocaine, quinidine, and barium. These agents also block a cell swelling-induced K conductance identified by macroscopic current measurements in the basolateral membranes of the intact colonic epithelium, suggesting that the 17 pS K channel identified by single-channel recording in isolated turtle colon cells may be responsible for this macroscopically defined K conductance.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Cell Physiology
Volume251
Issue number1
StatePublished - 1986
Externally publishedYes

Fingerprint

Turtles
Quinidine
Hyaluronoglucosaminidase
Potassium Channels
Clamping devices
Electric current measurement
Barium
Cell membranes
Lidocaine
Seals
Swelling
Colon
Epithelial Cells
Membranes
Glass
Electrodes
Patch-Clamp Techniques
Baths
Epithelium
Cell Membrane

ASJC Scopus subject areas

  • Cell Biology
  • Clinical Biochemistry
  • Physiology

Cite this

Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells. / Richards, N. W.; Dawson, D. C.

In: American Journal of Physiology - Cell Physiology, Vol. 251, No. 1, 1986.

Research output: Contribution to journalArticle

@article{ce3190ace31747fe882899f745a4050f,
title = "Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells",
abstract = "The patch-clamp technique for recording single-channel currents across cell membranes was applied to single turtle colon epithelial cells isolated with hyaluronidase. With electrodes fabricated from Corning #7052 glass, high-resistance seals were consistently formed to these cells. In on-cell patches with low K (2.5 mM) in the pipette and high K (114.5 mM) in the bath, outward K currents were recorded that had a slope conductance of 17 pS and a reversal potential greater than -70 mV. Currents through this K channel were blocked by lidocaine, quinidine, and barium. These agents also block a cell swelling-induced K conductance identified by macroscopic current measurements in the basolateral membranes of the intact colonic epithelium, suggesting that the 17 pS K channel identified by single-channel recording in isolated turtle colon cells may be responsible for this macroscopically defined K conductance.",
author = "Richards, {N. W.} and Dawson, {D. C.}",
year = "1986",
language = "English (US)",
volume = "251",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "1",

}

TY - JOUR

T1 - Single potassium channels blocked by lidocaine and quinidine in isolated turtle colon epithelial cells

AU - Richards, N. W.

AU - Dawson, D. C.

PY - 1986

Y1 - 1986

N2 - The patch-clamp technique for recording single-channel currents across cell membranes was applied to single turtle colon epithelial cells isolated with hyaluronidase. With electrodes fabricated from Corning #7052 glass, high-resistance seals were consistently formed to these cells. In on-cell patches with low K (2.5 mM) in the pipette and high K (114.5 mM) in the bath, outward K currents were recorded that had a slope conductance of 17 pS and a reversal potential greater than -70 mV. Currents through this K channel were blocked by lidocaine, quinidine, and barium. These agents also block a cell swelling-induced K conductance identified by macroscopic current measurements in the basolateral membranes of the intact colonic epithelium, suggesting that the 17 pS K channel identified by single-channel recording in isolated turtle colon cells may be responsible for this macroscopically defined K conductance.

AB - The patch-clamp technique for recording single-channel currents across cell membranes was applied to single turtle colon epithelial cells isolated with hyaluronidase. With electrodes fabricated from Corning #7052 glass, high-resistance seals were consistently formed to these cells. In on-cell patches with low K (2.5 mM) in the pipette and high K (114.5 mM) in the bath, outward K currents were recorded that had a slope conductance of 17 pS and a reversal potential greater than -70 mV. Currents through this K channel were blocked by lidocaine, quinidine, and barium. These agents also block a cell swelling-induced K conductance identified by macroscopic current measurements in the basolateral membranes of the intact colonic epithelium, suggesting that the 17 pS K channel identified by single-channel recording in isolated turtle colon cells may be responsible for this macroscopically defined K conductance.

UR - http://www.scopus.com/inward/record.url?scp=0022451693&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022451693&partnerID=8YFLogxK

M3 - Article

VL - 251

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 1

ER -