Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells

Tadayuki Akagi; Lee Yung Shih; Seishi Ogawa; Joachim Gerss; Stephen R. Moore; Rhona Schreck; Norihiko Kawamata; Der Cherng Liang; Masashi Sanada; Yasuhito Nannya; Stefan Deneberg; Vasilios Zachariadis; Ann Nordgren; Jee Hoon Song; Martin Dugas; Sören Lehmann; H. Phillip Koeffler

doi:10.3324/haematol.2009.005744

Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells

Tadayuki Akagi, Lee Yung Shih, Seishi Ogawa, Joachim Gerss, Stephen R. Moore, Rhona Schreck, Norihiko Kawamata, Der Cherng Liang, Masashi Sanada, Yasuhito Nannya, Stefan Deneberg, Vasilios Zachariadis, Ann Nordgren, Jee Hoon Song, Martin Dugas, Sören Lehmann, H. Phillip Koeffler

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

Translocation of chromosomes 8 and 21, t(8;21), resulting in the AML1-ETO fusion gene, is associated with acute myeloid leukemia. We searched for additional genomic abnormalities in this acute myeloid leukemia subtype by performing single nucleotide polymorphism genomic arrays (SNP-chip) analysis on 48 newly diagnosed cases. Thirty-two patients (67%) had a normal genome by SNP-chip analysis (Group A), and 16 patients (33%) had one or more genomic abnormalities including copy number changes or copy number neutral loss of heterozygosity (Group B). Two samples had copy number neutral loss of heterozygosity on chromosome 6p including the PIM1 gene; and one of these cases had E135K mutation of Pim1. Interestingly, 38% of Group B and only 13% of Group A samples had a KIT-D816 mutation, suggesting that genomic alterations are often associated with a KIT-D816 mutation. Importantly, prognostic analysis revealed that overall survival and event-free survival of individuals in Group B were significantly worse than those in Group A.

Original language	English (US)
Pages (from-to)	1301-1306
Number of pages	6
Journal	Haematologica
Volume	94
Issue number	9
DOIs	https://doi.org/10.3324/haematol.2009.005744
State	Published - Sep 2009
Externally published	Yes

Keywords

AML1-ETO
CNN-LOH
KIT
PIM1
SNP-chip
t(8;21)

ASJC Scopus subject areas

Hematology

Access to Document

10.3324/haematol.2009.005744

Cite this

Akagi, T., Shih, L. Y., Ogawa, S., Gerss, J., Moore, S. R., Schreck, R., Kawamata, N., Liang, D. C., Sanada, M., Nannya, Y., Deneberg, S., Zachariadis, V., Nordgren, A., Song, J. H., Dugas, M., Lehmann, S., & Koeffler, H. P. (2009). Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells. Haematologica, 94(9), 1301-1306. https://doi.org/10.3324/haematol.2009.005744

Akagi, T, Shih, LY, Ogawa, S, Gerss, J, Moore, SR, Schreck, R, Kawamata, N, Liang, DC, Sanada, M, Nannya, Y, Deneberg, S, Zachariadis, V, Nordgren, A, Song, JH, Dugas, M, Lehmann, S & Koeffler, HP 2009, 'Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells', Haematologica, vol. 94, no. 9, pp. 1301-1306. https://doi.org/10.3324/haematol.2009.005744

@article{3786497257cb4582971ac154e1810e39,

title = "Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells",

abstract = "Translocation of chromosomes 8 and 21, t(8;21), resulting in the AML1-ETO fusion gene, is associated with acute myeloid leukemia. We searched for additional genomic abnormalities in this acute myeloid leukemia subtype by performing single nucleotide polymorphism genomic arrays (SNP-chip) analysis on 48 newly diagnosed cases. Thirty-two patients (67%) had a normal genome by SNP-chip analysis (Group A), and 16 patients (33%) had one or more genomic abnormalities including copy number changes or copy number neutral loss of heterozygosity (Group B). Two samples had copy number neutral loss of heterozygosity on chromosome 6p including the PIM1 gene; and one of these cases had E135K mutation of Pim1. Interestingly, 38% of Group B and only 13% of Group A samples had a KIT-D816 mutation, suggesting that genomic alterations are often associated with a KIT-D816 mutation. Importantly, prognostic analysis revealed that overall survival and event-free survival of individuals in Group B were significantly worse than those in Group A.",

keywords = "AML1-ETO, CNN-LOH, KIT, PIM1, SNP-chip, t(8;21)",

author = "Tadayuki Akagi and Shih, {Lee Yung} and Seishi Ogawa and Joachim Gerss and Moore, {Stephen R.} and Rhona Schreck and Norihiko Kawamata and Liang, {Der Cherng} and Masashi Sanada and Yasuhito Nannya and Stefan Deneberg and Vasilios Zachariadis and Ann Nordgren and Song, {Jee Hoon} and Martin Dugas and S{\"o}ren Lehmann and Koeffler, {H. Phillip}",

year = "2009",

month = sep,

doi = "10.3324/haematol.2009.005744",

language = "English (US)",

volume = "94",

pages = "1301--1306",

journal = "Haematologica",

issn = "0390-6078",

publisher = "Ferrata Storti Foundation",

number = "9",

}

TY - JOUR

T1 - Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells

AU - Akagi, Tadayuki

AU - Shih, Lee Yung

AU - Ogawa, Seishi

AU - Gerss, Joachim

AU - Moore, Stephen R.

AU - Schreck, Rhona

AU - Kawamata, Norihiko

AU - Liang, Der Cherng

AU - Sanada, Masashi

AU - Nannya, Yasuhito

AU - Deneberg, Stefan

AU - Zachariadis, Vasilios

AU - Nordgren, Ann

AU - Song, Jee Hoon

AU - Dugas, Martin

AU - Lehmann, Sören

AU - Koeffler, H. Phillip

PY - 2009/9

Y1 - 2009/9

N2 - Translocation of chromosomes 8 and 21, t(8;21), resulting in the AML1-ETO fusion gene, is associated with acute myeloid leukemia. We searched for additional genomic abnormalities in this acute myeloid leukemia subtype by performing single nucleotide polymorphism genomic arrays (SNP-chip) analysis on 48 newly diagnosed cases. Thirty-two patients (67%) had a normal genome by SNP-chip analysis (Group A), and 16 patients (33%) had one or more genomic abnormalities including copy number changes or copy number neutral loss of heterozygosity (Group B). Two samples had copy number neutral loss of heterozygosity on chromosome 6p including the PIM1 gene; and one of these cases had E135K mutation of Pim1. Interestingly, 38% of Group B and only 13% of Group A samples had a KIT-D816 mutation, suggesting that genomic alterations are often associated with a KIT-D816 mutation. Importantly, prognostic analysis revealed that overall survival and event-free survival of individuals in Group B were significantly worse than those in Group A.

AB - Translocation of chromosomes 8 and 21, t(8;21), resulting in the AML1-ETO fusion gene, is associated with acute myeloid leukemia. We searched for additional genomic abnormalities in this acute myeloid leukemia subtype by performing single nucleotide polymorphism genomic arrays (SNP-chip) analysis on 48 newly diagnosed cases. Thirty-two patients (67%) had a normal genome by SNP-chip analysis (Group A), and 16 patients (33%) had one or more genomic abnormalities including copy number changes or copy number neutral loss of heterozygosity (Group B). Two samples had copy number neutral loss of heterozygosity on chromosome 6p including the PIM1 gene; and one of these cases had E135K mutation of Pim1. Interestingly, 38% of Group B and only 13% of Group A samples had a KIT-D816 mutation, suggesting that genomic alterations are often associated with a KIT-D816 mutation. Importantly, prognostic analysis revealed that overall survival and event-free survival of individuals in Group B were significantly worse than those in Group A.

KW - AML1-ETO

KW - CNN-LOH

KW - KIT

KW - PIM1

KW - SNP-chip

KW - t(8;21)

UR - http://www.scopus.com/inward/record.url?scp=70349116956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349116956&partnerID=8YFLogxK

U2 - 10.3324/haematol.2009.005744

DO - 10.3324/haematol.2009.005744

M3 - Article

C2 - 19734423

AN - SCOPUS:70349116956

SN - 0390-6078

VL - 94

SP - 1301

EP - 1306

JO - Haematologica

JF - Haematologica

IS - 9

ER -

Single nucleotide polymorphism genomic arrays analysis of t(8;21) acute myeloid leukemia cells

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this