Abstract
In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demonstrating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 65-72 |
| Number of pages | 8 |
| Journal | BMB Reports |
| Volume | 46 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2013 |
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Keywords
- Breast cancer
- ERBB2
- FISH
- Ribonucleoprotein (RNP)
- RNA
- Single-molecule imaging
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
Cite this
Single-molecule fluorescence in situ hybridization : Quantitative imaging of single RNA molecules. / Kwon, Sunjong.
In: BMB Reports, Vol. 46, No. 2, 02.2013, p. 65-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-molecule fluorescence in situ hybridization
T2 - Quantitative imaging of single RNA molecules
AU - Kwon, Sunjong
PY - 2013/2
Y1 - 2013/2
N2 - In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demonstrating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.
AB - In situ detection of RNAs is becoming increasingly important for analysis of gene expression within and between intact cells in tissues. International genomics efforts are now cataloging patterns of RNA transcription that play roles in cell function, differentiation, and disease formation, and they are demonstrating the importance of coding and noncoding RNA transcripts in these processes. However, these techniques typically provide ensemble averages of transcription across many cells. In situ hybridization-based analysis methods complement these studies by providing information about how expression levels change between cells within normal and diseased tissues, and they provide information about the localization of transcripts within cells, which is important in understanding mechanisms of gene regulation. Multi-color, single-molecule fluorescence in situ hybridization (smFISH) is particularly useful since it enables analysis of several different transcripts simultaneously. Combining smFISH with immunofluorescent protein detection provides additional information about the association between transcription level, cellular localization, and protein expression in individual cells.
KW - Breast cancer
KW - ERBB2
KW - FISH
KW - Ribonucleoprotein (RNP)
KW - RNA
KW - Single-molecule imaging
UR - http://www.scopus.com/inward/record.url?scp=84874740200&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84874740200&partnerID=8YFLogxK
U2 - 10.5483/BMBRep.2013.46.2.016
DO - 10.5483/BMBRep.2013.46.2.016
M3 - Article
C2 - 23433107
AN - SCOPUS:84874740200
VL - 46
SP - 65
EP - 72
JO - BMB Reports
JF - BMB Reports
SN - 1976-6696
IS - 2
ER -